A kind of method for extracting mangiferin

A technology of mangiferin and mango leaves, which is applied in the field of medicinal chemistry, can solve the problem that the content of active ingredients is less affected, and achieve the effects of short extraction cycle, reduced production cost, and simple operation

Active Publication Date: 2019-01-29
KPC PHARM INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current flash extraction can only increase the extraction speed and has little effect on the content of active ingredients

Method used

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  • A kind of method for extracting mangiferin
  • A kind of method for extracting mangiferin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, the extraction of mangiferin

[0018] Material: Mango leaf medicinal material (purchased in Xishuangbanna, Yunnan, batch number 20140615), after removing impurities, crushed into coarse powder.

[0019] Flash extraction: get 100g of mango leaf medicinal material coarse powder, add 10 times volume (W / V, by g / ml) 70% ethanol, extract 3 times with flash extractor, 5 minutes / time, filter, combine filtrate, Concentrate to dryness.

[0020] Ethanol reflux extraction: Take 100 g of the mango leaf medicinal material powder, add 10 times the volume (W / V, calculated in g / ml) of 70% ethanol for reflux extraction 3 times, 1 hour each time, filter, combine the filtrates, and concentrate to dryness.

Embodiment 2

[0021] Embodiment 2, the detection of mangiferin extraction rate

[0022] Taking the mangiferin content as an index, the determination was carried out according to high performance liquid chromatography (Appendix VID of the Chinese Pharmacopoeia, 2010 edition), and the dregs after extraction in Example 1 were respectively taken to measure the mangiferin residue, and the extraction transfer rate was calculated. The results are shown in Table 1.

[0023] Chromatographic conditions and system suitability test: octadecylsilane combined with silica gel as filler; methanol-0.2% phosphoric acid solution (30:70) as mobile phase; detection wavelength is 240nm. The number of theoretical plates calculated based on the mangiferin peak shall not be less than 3000.

[0024] Preparation of reference substance solution: Take an appropriate amount of mangiferin reference substance, weigh it accurately, add 50% methanol to make a solution containing 25 μg per 1 ml, and obtain it.

[0025] Pre...

Embodiment 3

[0030] Embodiment 3, the orthogonal test of the extraction process of extracting mangiferin

[0031] Material: Mango leaf medicinal material (purchased in Xishuangbanna, Yunnan, batch number 20140615), after removing impurities, crushed into coarse powder.

[0032] Take 100 g of mango leaf crude powder, perform flash extraction according to the conditions in Table 2, filter, take the extracted medicinal residues, measure the content after drying and calculate the transfer rate, and perform variance analysis according to the transfer rate under each extraction condition. The results are shown in Table 2, and the results of variance analysis are shown in Table 3.

[0033] Table 2 Extraction of mangiferin Orthogonal experiment results

[0034]

[0035]

[0036] Table 3 Orthogonal Extraction Experiment Analysis of Variance

[0037] factor

[0038] It can be seen from the results in the above table that under the conditions of several extraction factors, the numbe...

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Abstract

The invention belongs to the field of medicinal chemistry, and concretely discloses a method for extracting mangiferin. The method comprises the following steps: crushing mango leaves, adding a solvent, extracting the components by a flash extractor for 1-4 times, where each time lasts 3-15 minutes, filtering the components, merging filtrates, and concentrating the product to a dry state. A weight-to-volume ratio of a medicinal material to the solvent is 1:5-1:15 metered by g / ml. The method has the advantages of simple operation, fast extraction speed, short extraction period, saved energy consumption required by heating and cooling, and reduction of production cost. Compared with a traditional method, the purity of the mangiferin extracted by the invention is obviously higher, and the method is in favor of subsequent purification, is suitable for large-scale industrial production, and has high application value and exploitation prospect.

Description

technical field [0001] The invention belongs to the field of medicinal chemistry, and in particular relates to a method for extracting mangiferin. Background technique [0002] Mango (Mangifera indica L.) is one of the famous tropical fruits. In my country, mangoes are widely distributed in Yunnan, Guangxi, Guangdong, Hainan and other provinces, and the resources are very rich. Mango leaves are the leaves of mangoes. Mango leaves as medicinal materials are recorded in "National Chinese Herbal Medicine Compilation", "Guangxi Traditional Chinese Medicinal Materials Standard" 1990 edition, "Guangdong Provincial Traditional Chinese Medicinal Materials Standard" (2004 edition) and "Yunnan Provincial Traditional Chinese Medicinal Materials Standard". Modern pharmacological studies have shown that mangiferin is the main active ingredient of mango leaves, which has a variety of physiological effects, such as antitussive, expectorant, immune regulation, anti-inflammatory, analgesic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D407/04
Inventor 龚云麒张伟高小慧
Owner KPC PHARM INC
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