Pinctada martensii dunker antibody vital injection experimental method

A technology of Pinctada chinensis and experimental method, which is applied in the field of in vivo injection experiment of Pinctada japonica antibody, can solve the problems of inability to obtain shellfish cells, inability to further understand the in vivo effect, and limitation of mineralization function, and achieve stable experimental results. , low cost, easy to operate

Inactive Publication Date: 2010-05-26
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these studies lack the support of direct evidence. The main reason is that shellfish cells, especially nacre-secreting cells (mantle epithelial cells or their precursor cells) that can...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Select Hepu pearl oysters with a shell diameter of 4.5cm and a wet weight of 32g, and place them in artificial seawater with a pH value of 8.2 prepared by dissolving 6.67kg of sea salt in 200L of aerated tap water for 3 days to eliminate unhealthy ones. Individuals, healthy individuals were retained; the recombined soluble matrix protein of Hepu pinctadae was taken, and the soluble matrix protein was injected into New Zealand rabbits, and the mineralization-related soluble matrix protein antibody was prepared by the existing method, and the The antibody was purified; the purified antibody and the retained healthy individuals were taken separately, and the healthy individuals were divided into an injection group and a control group. Each injection group consisted of four healthy individuals, and 1.0 μg was injected according to the wet weight of 1 gram of healthy individuals per day. The ratio of the purified antibody, the purified antibody was injected into the body of h...

Embodiment 2

[0042]Select Hepu pearl oysters with a shell diameter of 6cm and a wet weight of 40g, and place them in artificial seawater with a pH value of 8.2 prepared by dissolving 6.67kg of sea salt in 200L of aerated tap water for 5 days, and eliminate unhealthy ones. Individuals, healthy individuals were retained; the recombined soluble matrix protein of Hepu Pinctada officinalis was taken, the soluble matrix protein was injected into New Zealand rabbits, and the soluble matrix protein antibody related to mineralization was prepared by the existing method, and the antibody Carry out purification; take the purified antibody and the retained healthy individuals respectively, divide the healthy individuals into an injection group and a control group, each injection group consists of four healthy individuals, and inject 1.0 μg of purified antibodies according to the wet weight of 1 gram of healthy individuals per day Ratio of antibodies after purification, the purified antibodies were inje...

Embodiment 3

[0044] Select Hepu pearl oysters with a shell diameter of 5.3cm and a wet weight of 36g, and place them in artificial seawater with a pH value of 8.2 prepared by dissolving 6.67kg of sea salt in 200L of aerated tap water for 4 days to eliminate unhealthy ones. Individuals, healthy individuals were retained; the recombined soluble matrix protein of Hepu pinctadae was taken, and the soluble matrix protein was injected into New Zealand rabbits, and the mineralization-related soluble matrix protein antibody was prepared by the existing method, and the The antibody was purified; the purified antibody and the retained healthy individuals were taken separately, and the healthy individuals were divided into an injection group and a control group. Each injection group consisted of four healthy individuals, and 1.0 μg was injected according to the wet weight of 1 gram of healthy individuals per day. The ratio of the purified antibody, the purified antibody was injected into the body of h...

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PUM

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Abstract

The invention relates to a pinctada martensii dunker antibody vital injection experimental method which comprises the following steps of: placing a pinctada martensii dunker into artificial seawater to be bred and retaining a healthy individual; preparing a soluble substrate protein antibody which is relevant to mineralization and purifying the antibody; injecting the purified antibody into a body of the injection group healthy individual according to the proportion and equivalently injecting nonimmune rabbit serum to a contrast group; separating, washing and soaking a shell of the injected healthy individual into a NaOH solution for eight minutes, washing and drying in the air; cutting off an area at the periphery of an injection point of the dried shell, sticking the area on a metal sheet and spraying metal powder on the surface of the shell; then placing the shell under an electronic scanning microscope, observing the abnormal growing condition of an innermost nacreous layer, confirming the influence of the antibody on the process of mineralization and further positioning the function of substrate protein in the process of the mineralization. The invention provides a research way for researching the mineralization of biological shellfish and an in-vivo factor and signal passage in vivo and provides a new idea for the research of a mineralization model.

Description

technical field [0001] The invention belongs to the technical field of in vivo experiments, and relates to a technique for in vivo research on the mineralization process of pearl oysters, in particular to an experimental method for in vivo injection of Hepu pearl oyster antibodies. Background technique [0002] Pinctada fucata Martenssi, also known as Pinctada fucata Martenssi, belongs to the phylum Mollusca, the class Bivalvia, the order Pterioida, the family Pteriidae, and the genus Pinctada ( Pinctada), mainly distributed in the coastal areas of my country, is currently the main shellfish used in the production of seawater pearls in the world. [0003] During the research on the molecular mechanism of nacre formation with Hepu pinnacle as the experimental material, a series of genes related to biomineralization were found, and related proteins involved in mineralization were obtained, but about these proteins in biomineralization The role of is not very clear. In additio...

Claims

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Application Information

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IPC IPC(8): A61K39/395
Inventor 张荣庆谢莉萍上官俊龙李琪刘晓军周玉娟
Owner TSINGHUA UNIV
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