Application of ophicalcitum extract and method for preparing same

A technology of stamens, extracts, applied in the fields of chemistry and medicine

Inactive Publication Date: 2012-12-05
于翔
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the cytotoxicity research on the water decoction of stamens (serpentine) raw products to cancer cells.

Method used

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  • Application of ophicalcitum extract and method for preparing same
  • Application of ophicalcitum extract and method for preparing same
  • Application of ophicalcitum extract and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: The preparation steps of stamen water extract and extract with anticancer activity:

[0016] Sampling: According to the requirements in "Rock and Mineral Analysis", use the interactive method and the quartering method to divide the stamens to ensure that the samples are uniform. Take out the stamens by quartering, wash and air-dry the stamens.

[0017] Pulverization: After the purified stamens are pulverized by a high-speed universal pulverizer, the powder is passed through a 100-200 mesh sieve and transferred to an oven, dried at a constant temperature of 105°C for 2 hours, and then placed in a drying oven to cool down for later use.

[0018] Extraction: Accurately weigh 100g of stamen powder dried at constant temperature, put it in a 2000mL round bottom flask, add 1000ml of deionized water at 1:10, reflux extraction 2-4 times, each time for 1-2h, filter while hot, and filter the filtrate Concentrate, set the volume in a 100ml volumetric flask, add water...

Embodiment 2

[0021] Embodiment 2 stamen (produced in Henan), serpentine fiber (produced in Sichuan) water decoction extract to human liver cancer cell HepG2, human liver cancer cell SMMC-7721, cervical cancer cell Hela, colon cancer cell SW-480 in vitro Cytostatic studies.

[0022] In vitro cancer cell inhibition experiments:

[0023] A: Prepare cancer cell suspension, take each tumor cell in logarithmic growth phase, digest with 0.25% trypsin, and prepare 5×10 cells with culture medium 4 cells / ml of cell suspension, seeded in 96-well culture plate, 100 μL per well.

[0024] B: Cells were cultured at 37°C, 5% CO2 and saturated humidity for 24 hours.

[0025] C: Filter the drug solution to be tested through a 0.45 μm filter membrane, add the drug according to the concentration of different crude drug amounts, 100 μL per hole, set 5 duplicate holes, use the normal cancer cell group as the negative control group, add the control group, etc. Measure fresh culture medium. Cells were culture...

Embodiment 3

[0038] Example 3 Study on the in vivo anticancer activity of stamen fiber nanoparticles on mouse liver tumor H22.

[0039] A: Culture and passage of H22 tumor cells

[0040]Before H22 cells were inoculated, they were mixed with DMEM+10% calf serum at 37°C and 5% CO 2 Subcultured to a good state, made 2×10 7 cell suspension per ml, inoculated in the peritoneal cavity of healthy Kunming mice, and passaged in ascites.

[0041] B: Establishment of H22 liver cancer mouse solid tumor model

[0042] Under aseptic conditions, extract the ascites of H22 mice inoculated for 7-9 days with a syringe, dilute with sterile saline to adjust the cell volume to 1×10 7 per ml, inoculated into the armpits of 50 mice, each with 0.2 ml, observed the growth of the mice, and made a solid tumor model.

[0043] C: Animal grouping and dosing

[0044] 24 hours after inoculation, they were randomly divided into 5 groups, 10 rats in each group, and administered by intragastric administration at regula...

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Abstract

The invention relates to application of an ophicalcitum extract and a method for preparing the same, and discloses a method for preparing ophicalcitum (with serpentine serving as a main component) extracting solution and anticancer application thereof. The extract can be extracted from ophicalcitum, and serpentine simple ore, serpentine-containing marble and rock having the characteristic of a serpentine ore family. The extract is originated from an ophicalcitum medicinal material, medicinal material submicron powder, mineral suspended particles and ophicalcitum natural nano-fiber thereof (the origins comprise raw / forged products). Researches on in-vivo and in-vitro pharmacological activities indicate that the ophicalcitum extract has very high anticancer activity and is expected to be developed into a new anticancer medicament using the ophicalcitum extract as a raw material and having wide clinical use value and commercial development value.

Description

technical field [0001] The invention relates to a preparation method of a traditional Chinese medicine stamen (serpentine) extract and its anticancer application, belonging to the technical fields of chemistry and medicine. Background technique [0002] The traditional Chinese medicine mineral stamenite is a kind of traditional Chinese medicine mineral medicine recorded in the 2005 edition of "Chinese Pharmacopoeia". "Jiayou Materia Medica", Dolomite "National Compilation of Chinese Herbal Medicine". It was first recorded in "Jiayou Materia Medica" in the Song Dynasty. "Materia Medica Yanyi" said: "There are pale white spots in the middle of the yellow stone, which is the name of the flower." In the valleys of the Cenozoic State (in Shanxi Province), there are five colors, which can replace cinnabar as a medicine. There are also Wenshan and Pengxian in the middle of Shu (Sichuan Province). In terms of textual research, it is consistent with the currently used stamen, that ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/10A61P35/00
Inventor 孟宪生卢秉久包永睿赵晶康廷国王海波
Owner 于翔
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