Magnetic immuno-chromatographic test paper strip for quantitatively detecting tumor associated antigen 19-9 in blood and preparation method thereof

Abstract

The invention relates to a magnetic immuno-chromatographic test paper strip for quantitatively detecting tumor associated antigen 19-9 in blood and a preparation method thereof. A coating film, a magnet particle pad combined with CA19-9 antibodies, a sample pad and a water absorbing pad are sequentially and alternately stuck on a base plate in a staggering way at intervals of 2mm, and then the upper layer is covered by a transparent plastic sealing film to form a test paper strip. A CA19-9 antibody detection line and a quality control line are pre-coated on the coating film. The invention introduces the magnetic immuno-chromatographic technology and the biotin-avidin system into the quantitative detection of CA19-9 in blood, and has the advantages that the detection sensitivity and the reliability are greatly improved, the detection is accurate and rapid, long-time waiting is not required, the cost is low, the operation method is simple and convenient and the detection can be controlled easily.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to a magnetic immunochromatographic test strip for quantitatively detecting tumor-associated antigen 19-9 in blood and a preparation method thereof. Background technique [0002] Tumor-associated antigen 19-9 is a tumor marker associated with pancreatic cancer, gallbladder cancer, colon cancer and gastric cancer, also known as pancreatic cancer-associated antigen (GICA). In 1979, Koprowski used the monosialoganglioside isolated from the surface of the human colon cancer cell line sw1116 as an antigen to make the corresponding monoclonal antibody 1116-Ns-19-9, which was used to identify The tumor-associated antigen is called CA19-9. CA19-9 is a mucus-type glycoprotein that exists in the blood and is composed of pancreatic cells, bile ducts, and epithelial cells of the stomach, colon, endometrium, and saliva. Serum cancer antigen 19-9 can be used as an auxiliary diagnostic index for p...

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Problems solved by technology

[0003] The current diagnostic techniques for CA19-9 in blood mainly include: radioimmunoassay (RIA), enzyme-linked immunoassay (ELISA), chemiluminescence (CLIA), time-resolved fluorescence and other methods, but these methods have their own characteristics and shortcomings : Radioimmunity is a commonly used method in clinical practice. Its advantages are relatively accurate results and a wide linear range. The disadvantages are that the operation is cumbersome and time-consuming, and radioactive markers will cause harm to the operator and cause environmental pollution. At present, it has been gradually adopted. replaced by other methods

Its appearance greatly simplifies people's detection behavior. However, due to the influence of various factors, immunochromatography technology can only achieve qualitative detection and semi-quantitative or inaccurate quantitative detection; the detection sensitivity of immunochromatography also needs to be improved. In particular, the widely used immunocolloidal gold chromatography test strips can only be judged by visual inspection, which is very subjective, and the results that different people may read are not small; the quantitative immunoassay technology based on photoelectric effect, due to It can only detect the light of immune particles on the surface of the nitrocellulose membrane, so it is difficult to truly reflect the total amount of immune particles on the detection line; in addition, it can be considered to set up multiple different detection lines on the nitrocellulose membrane at the same time to detect multiple joint detection lines at the same time In addition, due to the inhomogeneity of the nitrocellulose membrane, when the detection line or control line is drawn, the distribution of antibodies on the membrane is not uniform, resulting in the uneven distribution of immune particles on the membrane during detection, thus affecting the detection results

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