Immunochromatography assay and device taking color-developing agent as sample carrier and capable of repeated sample adding

An immunochromatography and color-developing reagent technology, applied in the field of immunochromatography, can solve the problems of decreased effective utilization rate, long time required to flow through the reaction zone, slow color reaction, etc., and achieves improved sensitivity and shortened interpretation time. Effect
CN101769924BActive Publication Date: 2013-03-20益思美诠生物科技(上海)有限公司
1 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
益思美诠生物科技(上海)有限公司
Publication Date
2013-03-20

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an immunochromatography assay and a device taking a color-developing agent as a sample carrier and capable of repeated sample adding, and the method comprises: 1) preparing a reagent strip according to the conventional method; 2) preparing the color-developing agent according to the conventional method, and mixing the corresponding antigen / antibody of a sample under test with the color-developing agent to form a color-developing agent mixture; 3) adding 50mu l-5ml color-developing agent mixture to a color-developing agent sample adding region; and 4) adding 5mu l-100mu l sample under test to the sample adding region when the color-developing agent enters a sample adding region of a nitrocellulose membrane, and reading the result within 1-30min, wherein, sample adding can be repeated in the meantime. Compared with other conventional methods, in the invention, the color-developing agent enters the nitrocellulose membrane earlier than the sample and has continuous follow-up supply, so the dynamic effects enable the invention to have the advantages of less amount of sample each time, shortened interpretation time, high sensitivity in unit time, clean background of the nitrocellulose membrane after reaction and the like.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to an immunochromatography method, in particular to an immunochromatography method and a device which use a chromogenic agent as a sample carrier and can add samples repeatedly. Background technique

[0002] In the past half century, most immunoassays have required expensive analytical equipment and instruments, and professionals have to operate the assays. Although these expensive detection methods have high sensitivity and specificity, they cannot meet the daily needs of people's health protection due to reasons such as expensive, inconvenient to carry, and professionalism.

[0003] Colloidal gold immunochromatography (GICA) technology is a simple and rapid immunological detection technology established on the basis of immunofiltration technology in the early 1990s. It was first used by Beggs et al. for human chorionic gonadotropin (HCG) determination. GICA uses nitrocellulose membrane (NC membrane) as the carrier, and uses th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More