Kit for detecting total bilirubin by using vanadate oxidation method

Abstract

The invention discloses a kit for detecting total bilirubin by using a vanadate oxidation method. The kit consists of a reagent 1 and a reagent 2 in the mixture ratio of 3:1. In the invention, by adding a compound, the solubility of vanadic acid is increased and cannot be reduced under the acid condition, thereby accelerating the reaction of the total bilirubin. In addition, an anti-interference substance is added in the kit to improve the capability of resisting an ascorbic acid interference reaction in a reaction process, so that the test result and a diazonium salt method have favorable clinical correlation. The kit has a better clinical application prospect.

Description

Technical field: [0001] The present invention relates to biological reagent technology. Specifically relates to a vanadate oxidation method detection kit for total bilirubin. Background technique: [0002] Bilirubin (bilirubin) is a metabolite of heme, which is mainly converted and excreted in the liver through conjugation; it can exist in three forms in serum: unconjugated bilirubin (BU, i.e. free bilirubin), conjugated bilirubin Bilirubin (Bc: sum of monoglucuronate Bcm and diglucuronate bilirubin Bcc) and bilirubin (albumin covalently linked to bilirubin). Bilirubin is an effective antioxidant with the ability to capture oxygen free radicals and protect lipids and lipoproteins from oxidation. It works synergistically with other antioxidant defense systems in the human body, and exists in the myocytes of the ventricle after being combined with albumin, preventing the generation of oxygen free radicals in this part, regulating the bilirubin antioxidant activity of myocard...

AI Technical Summary

Problems solved by technology

But it has defects: the first is that the diazonium reagent is unstable, it must be temporarily generated by sodium nitrite and aminobenzenesulfonic acid, and it can only be used for 2 days (generally 6 hours) after generation; The reaction specificity of gallbladder and total gallbladder is not good. When the total gallstone is measured under certain conditions, a part of the gallbladder will participate in the reaction. If the reaction time is prolonged, more will be involved, resulting in inaccurate measured values. Moreover, the operation of this method is complicated, The reagent is unstable and the reaction is not specific

[0008] Although the technology of measuring bilirubin by vanadate oxidation method has entered into clinical application, it is still not perfect. In the process of measuring total bilirubin, there is a problem that metavanadic acid is difficult to dissolve, and it is colloidal in the solution, making the reagent Elevated background

Especially under acidic conditions, the solubility will be greatly reduced, and metavanadate ions will be precipitated rapidly, which will increase the background of the solution and seriously affect the reaction.

If the concentration is lowered, the reaction will be incomplete and the measurement result will be lowered! Increasing the pH value of the reagent reaction will increase many side reactions, especially the interference of ascorbic acid on the reagent is particularly serious, seriously affecting the test results

However, the complete dissolution of vanadic acid requires a higher temperature, and the temperature on the automatic biochemical analyzer is generally 4-8 degrees. At this temperature, vanadic acid will immediately precipitate in the general buffer solution, which makes the detection impossible.

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