Kit for detecting total bilirubin by using vanadate oxidation method

A technology of total bilirubin and kits, applied in biological testing, material inspection products, etc., can solve the problems of solution background increase, poor reaction specificity, complicated operation, etc., to improve the ability of ascorbic acid to interfere with the reaction, Good clinical application prospect and good clinical relevance effect

Active Publication Date: 2010-07-14
SHANGHAI FOSUN PHARMA (GROUP) CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it has defects: the first is that the diazonium reagent is unstable, it must be temporarily generated by sodium nitrite and aminobenzenesulfonic acid, and it can only be used for 2 days (generally 6 hours) after generation; The reaction specificity of gallbladder and total gallbladder is not good. When the total gallstone is measured under certain conditions, a part of the gallbladder will participate in the reaction. If the reaction time is prolonged, more will be involved, resulting in inaccurate measured values. Moreover, the operation of this method is complicated, The reagent is unstable and the reaction is not specific
[0008] Although the technology of measuring bilirubin by vanadate oxidation method has entered into clinical application, it is still not perfect. In the process of measuring total bilirubin, there is a problem that metavanadic acid is difficult to dissolve, a

Method used

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  • Kit for detecting total bilirubin by using vanadate oxidation method
  • Kit for detecting total bilirubin by using vanadate oxidation method
  • Kit for detecting total bilirubin by using vanadate oxidation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Take two one-liter containers first, and add 0.8L of deionized water to each container;

[0042] 2. Add the substance of reagent 1 to container 1

[0043] Sodium citrate 29.4g, lactic acid 30g, FeCl2 0.001g, Triton x-1005ml, Tween 5ml sodium azide 0.5g;

[0044] 3. Add the substance of reagent 2 to container two

[0045] Na 2 CO 3 20g, ethanolamine 13.6g, ammonium metavanadate 0.5g, NaCl 1.2g, sodium azide 0.5g;

[0046] 4. The solution is fully stirred

[0047] 5. Finally, add the remaining 0.2L of water to both containers

[0048] 6. The reagent should be stored at room temperature of 20 degrees for 1 day and then stored in a refrigerator at 4 degrees.

[0049] Detected on the Olympus 400 detection instrument, the parameters are two-point endpoint method, the main wavelength is 450, the secondary wavelength is 540, the sample volume is 10 microliters, the reagent one is 280 microliters, and the reagent two is 70 microliters.

[0050] The kits of this exampl...

Embodiment 2

[0058] Reagent 1

[0059] 1. Take two one-liter containers first, and add 0.8L of deionized water to each container;

[0060] 2. Add the substance of reagent 1 to container 1

[0061] Glycine 7.5g, hydrochloric acid 5ml, FeCl2 500.002, Triton x-100 5ml, Tween 5ml, sodium azide 0.5g;

[0062] 3. Add the substance of reagent 2 to container two

[0063] Na 2 CO 3 18g, ethanolamine 14g, ammonium metavanadate 0.5g, NaCl 1.3g, sodium azide 0.5g;

[0064] 4. The solution is fully stirred

[0065] 5. Finally, add the remaining 0.2L of water to both containers

[0066] 6. The reagent should be stored at room temperature of 20 degrees for 1 day and then stored in a refrigerator at 4 degrees.

Embodiment 3

[0068] 1. Take two one-liter containers first, and add 0.8L of deionized water to each container;

[0069] 2. Add the substance of reagent 1 to container 1

[0070] Phthalic acid 15g, lactic acid 28g, FeCl2 0.010g, Triton x-100 5ml, Tween 5ml, sodium azide 0.5g;

[0071] 3. Add the substance of reagent 2 to container two

[0072] NaHCO 3 15g, ethanolamine 14g, lithium metavanadate 0.6g, NaCl 1.3g, sodium azide 0.5g;

[0073] 4. The solution is fully stirred

[0074] 5. Finally, add the remaining 0.2L of water to both containers

[0075] 6. The reagent should be stored at room temperature of 20 degrees for 1 day and then stored in a refrigerator at 4 degrees.

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Abstract

The invention discloses a kit for detecting total bilirubin by using a vanadate oxidation method. The kit consists of a reagent 1 and a reagent 2 in the mixture ratio of 3:1. In the invention, by adding a compound, the solubility of vanadic acid is increased and cannot be reduced under the acid condition, thereby accelerating the reaction of the total bilirubin. In addition, an anti-interference substance is added in the kit to improve the capability of resisting an ascorbic acid interference reaction in a reaction process, so that the test result and a diazonium salt method have favorable clinical correlation. The kit has a better clinical application prospect.

Description

Technical field: [0001] The present invention relates to biological reagent technology. Specifically relates to a vanadate oxidation method detection kit for total bilirubin. Background technique: [0002] Bilirubin (bilirubin) is a metabolite of heme, which is mainly converted and excreted in the liver through conjugation; it can exist in three forms in serum: unconjugated bilirubin (BU, i.e. free bilirubin), conjugated bilirubin Bilirubin (Bc: sum of monoglucuronate Bcm and diglucuronate bilirubin Bcc) and bilirubin (albumin covalently linked to bilirubin). Bilirubin is an effective antioxidant with the ability to capture oxygen free radicals and protect lipids and lipoproteins from oxidation. It works synergistically with other antioxidant defense systems in the human body, and exists in the myocytes of the ventricle after being combined with albumin, preventing the generation of oxygen free radicals in this part, regulating the bilirubin antioxidant activity of myocard...

Claims

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Application Information

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IPC IPC(8): G01N33/72
Inventor 景晟张跃建
Owner SHANGHAI FOSUN PHARMA (GROUP) CO LTD
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