The invention discloses a compound bifonazole nano-emulsion spray preparation. The nano-emulsion spray preparation has the grain size of 1 nm to 100 nm and is formed by the following raw materials in mass percent: 30%-40% of surfactant, 3%-14% of cosurfactant, 5%-10% of oil phase, 0.8%-2% of bifonazole, 0.02%-0.1% of clobetasol propionate, 0.01%-0.2% of cinnamyl aldehyde and the balance of deionized water, wherein the sum of the mass percents of the raw materials is 100%. The compound bifonazole nano-emulsion spray preparation has multiple actions, such as fungicide, bactericide, anti-bacterial, anti-fungal, antiinflammation, itching relieving and the like, treats superficial skin fungous infection, fungous paronychia and candidal vulvovaginitis, and is also effective to external otitis and bacterial skin infection. The compound bifonazole nano-emulsion spray preparation prepared by a preparation method of the compound bifonazole nano-emulsion spray preparation has the advantages that: the activity of the three drugs is remarkably improved, the effect of sterilization and itching relieving is rapid, the recurrence is difficult, and the preparation method is simple, so that the compound bifonazole nano-emulsion spray preparation and the preparation method thereof have broad market prospects in the medicine field.