Gene transformation method of oil sunflower

Abstract

The invention relates to a gene transformation method of oil sunflower, which comprises the following steps of (1) taking the tissue of a test tube plantlet of the robustly-growth oil sunflower as an explant; (2) inoculating one single bacterial colony of agrobacterium tumfaciens C58C1 into an LB liquid nutrient medium for shake cultivation overnight, taking out the bacterial colony after the OD value is 0.2-0.6 and impregnating the explant of the tissue of the test tube plantlet of the oil sunflower; (3) placing the impregnated explant of the tissue of the test tube plantlet of the oil sunflower on a differential medium containing 2-5mg / L of herbicide for differential cultivation for sub-culturing every time within 0-14 days so as to generate an inversion plantlet; (4) directly inverting the inversion plantlet screened by the herbicide into a rooting medium and transplanting the inversion plantlet into a flower pot when the inversion plantlet grows at 2-5cm. The invention establishes the set of the gene transformation method of the oil sunflower, so as to lead the steps of screening the marking concentration (the herbicide), choosing different inversion explants and adopting different sub-culturing times to be clear respectively and obtain the highest inversion efficiency, thereby laying the foundation for various gene transformations of the oil sunflower.

Description

technical field

[0001] The invention belongs to the field of agriculture and plant breeding, in particular to a method for gene transformation of sunflower. Background technique

[0002] Sunflower for oil (abbreviated oil sunflower) is one of the world's three major oil crops with the fastest total output growth in the past 30 years, with an annual growth rate of 7.1%. The main producing countries of oil sunflower in the world are Argentina, France, followed by China. my country's oil sunflowers are mainly concentrated in the three northeastern provinces, Inner Mongolia, Xinjiang, and the rapidly developing provinces and autonomous regions of Shaanxi, Shanxi, and Hebei. The oil content of sunflower seeds is generally about 46%, the highest can reach more than 50%, and the oil yield is more than 42%. Sunflower oil does not contain erucic acid, glucosinolate and other substances harmful to the human body, but it is rich in unsaturated fatty acids that are very beneficial to t...