Tissue culture rapid propagation method of Rhizoma Typhonii Flagelliformis
A technology of tissue culture rapid propagation and water pinellia, applied in the field of plant cell engineering, can solve the problems of long time (it takes about 3 months, difficult callus, etc.), and achieve the effect of increasing speed, high economic value, and consistent traits
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Embodiment 1
[0034] Embodiment 1, a kind of tissue culture rapid propagation method of Pinellia chinensis, carries out the following steps successively:
[0035] 1), fresh water pinellia tubers are washed with clear water, soaked in alcohol with a volume concentration of 75% for 0.5 to 1 min, rinsed with sterile water for 3 to 4 times, and then disinfected with a mercuric chloride aqueous solution with a mass concentration of 0.1% for 10 min. Rinse with bacterial water 6-8 times.
[0036] Cut off the cortex of the above-mentioned sterilized Pinellia tuber, and then cut it into 0.3-0.5 cm under aseptic conditions. 2 (The purpose is to remove the tissue treated with mercuric chloride so that the fresh tissue can contact the medium), form a tuber I, and inoculate it on the differentiation medium. Configure 25-30ml of differentiation medium in a tissue culture bottle with a capacity of 250ml, place 4-5 tubers I in each bottle, and cultivate under light at a temperature of 25±4°C. The light ti...
Embodiment 2
[0045] Get fresh Pinellia tuber, concrete steps are as shown in embodiment 1, difference is that the culture temperature of inducing the organ differentiation of Pinellia is 21 ℃, grows bud in 23~26 days, each explant (tuber 1) emerges 4 to 6 buds;
[0046] The 30-day multiplication coefficient is 1:10-20, and the 90-day multiplication coefficient is 1:80-120. The 120-day multiplication coefficient is 1:650-900.
Embodiment 3
[0048] Get the clustered buds of fresh Pinellia chinensis, the specific steps are as shown in Example 1, the difference is that the culture temperature for inducing the organ differentiation of Pinellia shuifu is 29°C, and after 14 days, 4 to 6 buds are produced on each tuber 1 ;
[0049] The multiplication factor for 30 days is 1:10~18, the multiplication factor for 90 days is 1:75~90, and the multiplication factor for 120 days is 1:550~750.
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