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Tissue culture rapid propagation method of Rhizoma Typhonii Flagelliformis

A technology of tissue culture rapid propagation and water pinellia, applied in the field of plant cell engineering, can solve the problems of long time (it takes about 3 months, difficult callus, etc.), and achieve the effect of increasing speed, high economic value, and consistent traits

Inactive Publication Date: 2010-08-18
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above techniques all form regenerated plants by establishing a callus system. Generally speaking, it is relatively difficult to induce callus and takes a long time (about 3 months)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, a kind of tissue culture rapid propagation method of Pinellia chinensis, carries out the following steps successively:

[0035] 1), fresh water pinellia tubers are washed with clear water, soaked in alcohol with a volume concentration of 75% for 0.5 to 1 min, rinsed with sterile water for 3 to 4 times, and then disinfected with a mercuric chloride aqueous solution with a mass concentration of 0.1% for 10 min. Rinse with bacterial water 6-8 times.

[0036] Cut off the cortex of the above-mentioned sterilized Pinellia tuber, and then cut it into 0.3-0.5 cm under aseptic conditions. 2 (The purpose is to remove the tissue treated with mercuric chloride so that the fresh tissue can contact the medium), form a tuber I, and inoculate it on the differentiation medium. Configure 25-30ml of differentiation medium in a tissue culture bottle with a capacity of 250ml, place 4-5 tubers I in each bottle, and cultivate under light at a temperature of 25±4°C. The light ti...

Embodiment 2

[0045] Get fresh Pinellia tuber, concrete steps are as shown in embodiment 1, difference is that the culture temperature of inducing the organ differentiation of Pinellia is 21 ℃, grows bud in 23~26 days, each explant (tuber 1) emerges 4 to 6 buds;

[0046] The 30-day multiplication coefficient is 1:10-20, and the 90-day multiplication coefficient is 1:80-120. The 120-day multiplication coefficient is 1:650-900.

Embodiment 3

[0048] Get the clustered buds of fresh Pinellia chinensis, the specific steps are as shown in Example 1, the difference is that the culture temperature for inducing the organ differentiation of Pinellia shuifu is 29°C, and after 14 days, 4 to 6 buds are produced on each tuber 1 ;

[0049] The multiplication factor for 30 days is 1:10~18, the multiplication factor for 90 days is 1:75~90, and the multiplication factor for 120 days is 1:550~750.

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Abstract

The invention discloses a tissue culture rapid propagation method of Rhizoma Typhonii Flagelliformis, which sequentially comprises the following steps that: 1) after being sterilized, tubers of Rhizoma Typhonii Flagelliformis is cut into a tuber I to be inoculated on a differential medium; 2) when a bud with the height of 0.1 to 0.3 cm is grown on the tuber I, the tuber I is cut into tubers II with 1 to 2 buds, and the tuber II is inoculated on a cluster bud culture medium; 3) when the cluster bud on the rubber II is grown to 3 to 4 cm, the tuber II is transplanted to a root culture medium to be cultured; 4) when the entire height of the Rhizoma Typhonii Flagelliformis is 5 to 7 cm, the Rhizoma Typhonii Flagelliformis plant which can be planted can be obtained through conventional outdoor seedling culture method. With the method, a great amount of Rhizoma Typhonii Flagelliformis plants can be rapidly obtained.

Description

technical field [0001] The invention belongs to the technical field of plant cell engineering, mainly utilizes plant tissue culture technology, and specifically relates to a method for large-scale tissue culture and rapid propagation of Shui Pinellia. Background technique [0002] Water Pinellia [Typhonium flagelliforme (Lodd.) Blume] is a plant of the genus Araceae, and its tuber is an important Chinese herbal medicine. Shui Pinellia has the effects of drying dampness and reducing weight, reducing swelling and pain, clearing away heat and detoxification, etc. It is generally used to treat cough, phlegm, sores, carbuncles, boils, bruises and traumatic bleeding. Water Pinellia was originally a folk drug in Guangdong and Guangxi. Although it was recorded in the "Chinese Pharmacopoeia" in recent decades, it is now well known and used because of its medicinal efficacy similar to that of Pinellia. It was introduced and cultivated as a wild species in the early 1960s, and it has ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈集双张立红丁伟田庆常
Owner ZHEJIANG SCI-TECH UNIV
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