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Method for tissue culture and rapid propagation of narcissus pseudonarcissus by using ramentum

A fast and narcissistic technology, applied in the field of agriculture, to achieve the effect of increasing the speed of reproduction, shortening the production cycle and saving costs

Inactive Publication Date: 2011-11-16
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been reports on tissue culture and rapid propagation of explants using European daffodil scale leaf axillary buds in China, but tissue culture and rapid propagation of explants using scales have not been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] European narcissus tissue culture and rapid propagation method are carried out in several steps in the following order:

[0057] 1. Selection and sterilization of explant parts: 2-year-old Narcissus bulbs were stored at 5°C for 40 days. Choose a bulb with a strong appearance, peel off the outermost layer of dry scales and residual old roots at the base at room temperature, soak it in washing powder water for 5 minutes as usual, rinse it with running water for 30 minutes, and then soak it in 70% (volume percentage) alcohol for 1 minute. After washing 4 times with sterile water, remove the upper 1 / 3 scale leaves, and then put 0.1% (mass percentage) HgCl 2 Soak in the solution for 10 minutes, and then rinse with sterile water 4 times as a spare material for primary culture. The treated bulbs are first removed from the bulb disk, and then the scales are cut into blocks of 10mm×10mm.

[0058] 2. Callus induction culture: Inoculate the lower part of the scales into the call...

Embodiment 2

[0065] This European narcissus tissue culture and rapid propagation method, except for the following distinguishing features, all the other steps and methods are the same as in Example 1.

[0066] The explants used in the first step are 2-year-old European narcissus bulbs, which have been treated at a low temperature of 9°C for 45 days, and the inner and lower scales of the bulbs are selected.

[0067] The callus induction medium in the second step is MS+6-BA 2.0mg L -1 +2,4-D 0.3mg L -1 + sucrose 30g / L+ agar 5.6g / L, pH 5.8, culture temperature 25°C, light time 12h / d, light intensity 1000lx, culture 30d, callus induction rate 98%.

[0068] Adventitious bud or bulblet differentiation medium in step 3 is MS+6-BA 2.0mg·L -1 +NAA0.2mg·L -1 + sucrose 30g / L+ agar 5.6g / L, pH 5.8, culture temperature 25°C, light intensity 2000lx, light time 12h / d, cultured for 35 days, the induced differentiation rate was 96%.

[0069] The subculture medium of step 4 is MS+6-BA 1.5mg L -1 +NAA0.3...

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PUM

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Abstract

The invention relates to a method for the tissue culture and rapid propagation of narcissus pseudonarcissus by using ramentum. The method comprises the following steps of: taking the ramentum of the narcissus pseudonarcissus which is 1 to 2 years old as an explant; performing the steps of induction, differentiation, secondary multiplication, sound seedling, rooting, transplanting and the like; and finally obtaining commercialized narcissus pseudonarcissus seedlings. The method of the invention has the advantages of high direction, high adaptability, simple and practical operation, achievementin up to 10 induced propagation multiple, over 95 percent of rooting rate and over 95 percent of survival rate of transplanting, and application in industrial production.

Description

technical field [0001] The invention relates to a new method for cultivating and rapidly propagating European narcissus by using scale tissue, and belongs to the field of agricultural technology. technical background [0002] European narcissus (Narcissus pseudonarcissus L.), also known as narcissus, is a general term for new species of narcissus introduced from Europe to my country, and is a perennial herb of the genus Narcissus in the family Amaryllidaceae. Compared with Chinese narcissus, European narcissus has the characteristics of large and colorful flowers and wide application. Its flower diameter is 3 to 5 times that of Chinese narcissus, and its bright colors are very popular in the domestic market. After the 1980s, large-scale introductions were carried out, mainly for spring flower exhibitions, and a small amount of potted plants were used as New Year's Eve flowers. European narcissus is an excellent garden ground cover flower. It can be used as potted ornamental...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王小敏李维林吴文龙李海燕闾连飞
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI