The invention relates to an automobile tensioner assembly performance testing mechanism and a working method thereof, and belongs to the field of automobile tensioner performance testing. The testing mechanism comprises a bottom plate, an output line, a tray mechanism, a jacking mechanism, a servo electric cylinder, an electric cylinder fixing plate, a pressure head fixing plate, a first air cylinder, a first air cylinder mounting plate, a second air cylinder, a second air cylinder mounting plate, a force measuring pressure head piece, a hanging component and a jacking pawl mechanism, the output line is mounted on the bottom plate, the tray mechanism is arranged on the output line, the jacking mechanism is connected with the tray mechanism, the servo electric cylinder is arranged on the electric cylinder fixing plate and is connected with the pressure head fixing plate, the first air cylinder is fixed to the first air cylinder mounting plate, the second air cylinder is fixed to the second air cylinder mounting plate, the force measuring pressure head piece is installed on the pressure head fixing plate and connected with the servo electric cylinder, the hanging component is fixed to the first air cylinder, and the pawl ejecting mechanism is connected with the second air cylinder. The testing mechanism is simple and reasonable in structural design, accurate in test, convenient to operate, high in automation degree and capable of meeting performance test requirements.