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Method for preparing suspension culture cell line of gynura bicolor embryonic cells and special culture medium thereof

A technique for culturing cells in suspension and S. japonica, applied to plant cells and other directions, can solve the problems of hindering the industrial production and application of S. japonica flavonoids, and having no embryogenic cell suspension system of S. japonica, achieving The effect of vigorous cell division, good dispersion and bright yellow color

Inactive Publication Date: 2013-05-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, there is no method for establishing a suspension line of embryogenic cells of G. purple-backed, which also hinders the industrial production and application of flavonoids such as anthocyanins contained in G. purple-backed

Method used

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  • Method for preparing suspension culture cell line of gynura bicolor embryonic cells and special culture medium thereof
  • Method for preparing suspension culture cell line of gynura bicolor embryonic cells and special culture medium thereof
  • Method for preparing suspension culture cell line of gynura bicolor embryonic cells and special culture medium thereof

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Embodiment 1

[0027] Embodiment 1, prepare the germogen cell suspension culture cell line of G. violet back

[0028] 1. Obtaining and Statistical Observation of Embryogenic Callus

[0029] 1. Pretreatment of explants

[0030] Select tender and flat leaves of Gelonia purple back (the variety is red leaves, purchased from the Vegetable Center of Beijing Academy of Agriculture and Forestry Sciences) as explants, first sterilize in 70% (volume percentage) alcohol for 30 seconds, and rinse with sterile water for 30 seconds. Once again, soak for 8 min with 0.1% (volume percentage) of Tween added dropwise with mercuric chloride, then rinse with sterile water.

[0031] 2. Acquisition of embryogenic callus

[0032] 1) Induction of callus

[0033] Cut the leaves of G. purpurea pretreated in the above step 1 into small pieces of about 1 cm × 1 cm, inoculate them on the callus induction medium with the back of the leaves facing down, at a temperature of 26 ° C, and a light intensity of 2500 lux. Th...

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Abstract

The invention discloses a method for preparing a suspension culture cell line of gynura bicolor embryonic cells and a special culture medium thereof. In the invention, a liquid culture medium is prepared by adding 2,4-D, KT and a carbon source in MS basic culture solution; and in the liquid culture medium, the final concentration of the 2,4-D is 1 to 3 mg / L, and the final concentration of KT is 0.2 to 1mg / L. In the method for preparing the suspension culture cell line of the gynura bicolor embryonic cells of the invention, gynura bicolor embryonic calluses are cultured in the liquid culture medium of the invention to obtain the suspension culture cell line. In the invention, the induction rate of the calluses is up to 100 percent. The method can effectively prepare the suspension culture cell line of the gynura bicolor embryonic cells and lays a reliable foundation for the construction of a bioreactor using gynura bicolor as a raw material.

Description

technical field [0001] The invention relates to the field of cell suspension culture, in particular to a method for preparing a cell line of suspension culture of germinal cells of G. purple backs and a special medium thereof. Background technique [0002] Gynura bicolor, scientific name Gynura bicolor DC, also known as purple back vegetable, two-color Sanqi grass, red back vegetable, Guanyin vegetable, is a perennial herbaceous plant of the genus Panax notoginseng in the Compositae family. The whole plant is purple-green, with long egg-shaped leaves and serrated edges. The leaf surface is green and slightly purple, and the back is purple-red waxy and shiny. It is a wild vegetable with medicinal value. As a high-nutrition and health-care vegetable worthy of promotion, Gastronia violet contains relatively rich and comprehensive nutrients. In addition to the nutrients of ordinary vegetables, it is also rich in nutrients that can improve animal disease resistance, and has a mod...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 郭仰东刘莉莎赵立群赵永钦温常龙王玉珏程琳
Owner CHINA AGRI UNIV