Application of thiospirolactone in intracellular hypochloric acid detection

Abstract

The invention relates to a fluorescence detection method and provides a fluorescent probe which has high sensitivity and high selectivity and is used for detecting intracellular hypochloric acid and an application of thiospirolactone in intracellular hypochloric acid detection. The structure of the fluorescent probe for detecting the intracellular hypochloric acid is a triphenylmethane fluorescent probe with a Xanthone ring parent structure; and the fluorescent probes of the thiospirolactone all adopt corresponding spirolactones as raw materials. The thiospirolactone has high-sensitivity fluorescent response characteristics on hypochloric acid, and when being used in the fluorescent probe for detecting the intracellular hypochloric acid, the selectivity is high. The probe has strong lightstability, no illumination self oxidation, rapid response to an aimed compound, good cell permeability and no strong cytotoxicity, and a product formed by combining the fluorescent probe and the aimed compound has strong photobleaching resistance. The thiospirolactone used for detecting the hypochloric acid of the aimed compound has high sensitivity and strong specificity and other active oxygen in cells has no interference on the detection.

Description

technical field [0001] The invention relates to a fluorescence detection method, in particular to a method for using thiospirolide as a fluorescent probe to detect hypochlorous acid (HClO), an active oxygen species produced in cells. Background technique [0002] Active oxygen is a general term for a series of oxygen-containing substances with active chemical properties and strong oxidizing ability produced by organisms. Active oxygen includes not only some free radicals, such as hydroxyl radical (OH), superoxide anion (O 2 - ), peroxyl radicals (ROO ), etc., also include some non-free radicals, such as hydrogen peroxide (H 2 o 2 ), singlet oxygen ( 1 o 2 ) and hypochlorous acid (HOCl), etc. [0003] The appropriate amount of reactive oxygen species spontaneously produced by organisms plays an important positive role in various life activities such as signal conversion, neurotransmission, immune system control, and synthesis and metabolism of important physiologically ...

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Problems solved by technology

Among the other reported HClO probes, some need harsh operating conditions at pH 12 (document: H.M.Ma, Chem.Eur.J.2008, 14, 4719.), or poor water solubility (document: W.Tan, et al, Chem.Eur.J.2009, 15, 2305.), cannot really be applied in biological systems

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