Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of thiospirolactone in intracellular hypochloric acid detection

A technology of hypochlorous acid and cells, which is applied in the field of fluorescence detection, can solve problems such as poor water solubility, inability to use biological systems, and harsh operating conditions, and achieve the effects of high selectivity, strong photobleaching resistance, and high sensitivity

Inactive Publication Date: 2010-10-27
XIAMEN UNIV
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the other reported HClO probes, some need harsh operating conditions at pH 12 (document: H.M.Ma, Chem.Eur.J.2008, 14, 4719.), or poor water solubility (document: W.Tan, et al, Chem.Eur.J.2009, 15, 2305.), cannot really be applied in biological systems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of thiospirolactone in intracellular hypochloric acid detection
  • Application of thiospirolactone in intracellular hypochloric acid detection
  • Application of thiospirolactone in intracellular hypochloric acid detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] The chemical properties of the fluorescent probe for detecting intracellular hypochlorous acid described in the present invention are described as follows.

[0036] 1. Chemical performance test of fluorescent probe in aqueous solution in vitro:

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a fluorescence detection method and provides a fluorescent probe which has high sensitivity and high selectivity and is used for detecting intracellular hypochloric acid and an application of thiospirolactone in intracellular hypochloric acid detection. The structure of the fluorescent probe for detecting the intracellular hypochloric acid is a triphenylmethane fluorescent probe with a Xanthone ring parent structure; and the fluorescent probes of the thiospirolactone all adopt corresponding spirolactones as raw materials. The thiospirolactone has high-sensitivity fluorescent response characteristics on hypochloric acid, and when being used in the fluorescent probe for detecting the intracellular hypochloric acid, the selectivity is high. The probe has strong lightstability, no illumination self oxidation, rapid response to an aimed compound, good cell permeability and no strong cytotoxicity, and a product formed by combining the fluorescent probe and the aimed compound has strong photobleaching resistance. The thiospirolactone used for detecting the hypochloric acid of the aimed compound has high sensitivity and strong specificity and other active oxygen in cells has no interference on the detection.

Description

technical field [0001] The invention relates to a fluorescence detection method, in particular to a method for using thiospirolide as a fluorescent probe to detect hypochlorous acid (HClO), an active oxygen species produced in cells. Background technique [0002] Active oxygen is a general term for a series of oxygen-containing substances with active chemical properties and strong oxidizing ability produced by organisms. Active oxygen includes not only some free radicals, such as hydroxyl radical (OH), superoxide anion (O 2 - ), peroxyl radicals (ROO ), etc., also include some non-free radicals, such as hydrogen peroxide (H 2 o 2 ), singlet oxygen ( 1 o 2 ) and hypochlorous acid (HOCl), etc. [0003] The appropriate amount of reactive oxygen species spontaneously produced by organisms plays an important positive role in various life activities such as signal conversion, neurotransmission, immune system control, and synthesis and metabolism of important physiologically ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/64C07D495/10
Inventor 詹心琪郑洪许金钩
Owner XIAMEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com