Paste dosage-form trichoderma biopesticide composite and preparation method thereof
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A biopesticide and composition technology, applied in botany equipment and methods, chemicals for biological control, biocides, etc., can solve the problems of insufficient storage time and unstable preparation quality, and achieve environmental protection , stable product quality and long storage time
Active Publication Date: 2010-11-03
JIANGSU GENGYUN CHEM CO LTD
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[0002] At present, most of the dosage forms of biological pesticides are powder, wettable powder, suspension concentrate, emulsifiable concentrate, water preparation, egg card, live insec...
Method used
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Embodiment 1
[0025] Example 1, a biopesticide composition in paste form and its preparation method: the ingredients of the present invention are: Trichoderma mycelia, Trichoderma chlamydospores, hydrochloric acid, potato starch and copper ions.
[0026] Adopt Trichoderma liquid culture medium formula 1: sugar beet liquid 20.0g, (NH 4 ) 2 SO 4 0g, MgSO 4 ·7H 2 O0.2g, KH 2 PO 4 2.0g, sucrose 0g;
[0027] Trichoderma was inoculated by 5% inoculum, at 26 ° C, shake cultured at 150 rpm / min for 36 hours, filtered, the filtered mycelium was taken out, the pH of the mycelia was adjusted to 3.5 with hydrochloric acid, and a small amount of copper ion inhibitor was added, and then Add 10% potato starch and stir evenly to prepare the bio-pesticide in paste form. The preparation process needs to be operated under sterile conditions.
Embodiment 2
[0028] Example 2 is basically the same as Example 1, but it is inoculated into a sterile liquid medium with an inoculum size of 2%; it is shaken at 28° C. for 48 hours, and after the filtered mycelium is taken out, the pH of the mycelium is adjusted to 3.0, add a small amount of copper ion inhibitor, and then add 8% potato starch.
[0029] Trichoderma liquid medium formula is: sugar beet liquid 20.0g, (NH 4 ) 2 SO 4 0g, MgSO 4 ·7H 2 O 0.2g, KH 2 PO 4 2.0g, 0g of sucrose.
Embodiment 3
[0030] Example 3 is basically the same as Example 1, but is inoculated into a sterile liquid medium with an inoculum size of 10%; at 27° C., shaking culture for 40 h, after the filtered mycelium is taken out, the pH of the mycelia is adjusted with hydrochloric acid To 4.0, add a small amount of copper ion inhibitor, and then add 5% potato starch.
[0031] Trichoderma liquid medium formula is: sugar beet liquid 20.0g, (NH 4 ) 2 SO 4 0g, MgSO 4 ·7H 2 O 0.2g, KH 2 PO 4 2.0g, 5.0g of sucrose.
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Abstract
The invention relates to a paste dosage-form trichoderma biopesticide composite and a preparation method thereof. The paste dosage-form trichoderma biopesticide composite comprises pesticide components and auxiliary materials, wherein the pesticide components include trichoderma hyphae and trichoderma chlamydospores, wherein the trichoderma chlamydospores are dispersed in the trichoderma hyphae. The paste dosage-form trichoderma biopesticide composite is characterized in that the auxiliary materials are starches which account for 5-10 percent of the total weight of the paste dosage-form trichoderma biopesticide composite. The preparation method comprises the following steps of: inoculating the trichoderma strains into a sterile liquid culture medium to carry out shake culture for 36-48 hours; taking out filtered trichoderma hyphae; regulating the pH of the trichoderma hyphae by using a hydrochloric acid; and adding a copper ion inhibitor and 5-10 percent of the starches for uniformly stirring so as to prepare the paste dosage-form trichoderma biopesticide composite. The paste dosage-form trichoderma biopesticide composite and a paste dosage-form biopesticide prepared by the methodhave the maximal disadvantages of stable product quality and long saving time, can be stored for 18 months at normal temperature and kept in high activity and can be stored for 3 years under the condition of refrigeration; and secondly, the invention has rarefied formula and advanced process and is beneficial to environmental protection without generating any waste water, waste gas and waste residues.
Description
technical field [0001] The present invention relates to a kind of biopesticide and its preparation method, especially a kind of trichoderma biopesticide composition of paste form, and the present invention will also provide the preparation method of this paste form of trichoderma biopesticide composition. Background technique [0002] At present, most of the dosage forms of biological pesticides are powder, wettable powder, suspension concentrate, emulsifiable concentrate, water preparation, egg card, live insects, etc. Although they have certain control effects on diseases, insect pests and weeds, the storage time is not long enough, and the quality of the preparations is also low. Not stable enough. Contents of the invention [0003] The object of the present invention is to provide a kind of Trichoderma biopesticide composition of pasty dosage form and preparation method thereof, the Trichoderma biopesticide composition of this pasty dosage form can well prevent and tre...
Claims
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