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Steroid saponin compounds and preparation method and application thereof

A technology for steroidal saponins and compounds is applied in the fields of steroidal saponin compounds and their preparation and application, and achieves the effects of low cost, high yield and good effect

Inactive Publication Date: 2010-11-03
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recent studies have shown that the above-mentioned effects of saponins may be related to their ability to increase the content of sex hormones in the body

Method used

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  • Steroid saponin compounds and preparation method and application thereof
  • Steroid saponin compounds and preparation method and application thereof
  • Steroid saponin compounds and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the preparation of steroidal saponin compound

[0043] Tribulus terrestris was purchased from Shenzhen Research Center of Traditional Chinese Medicine, Guangdong Province, the product catalog number is TT2002061211.

[0044] 1. Preparation

[0045] 1. Take 2.5kg of Tribulus terrestris and pulverize it, and extract it with 55L of 70% ethanol (ethanol:water=70:30, volume ratio). The extraction steps are as follows: cold soak at 25°C (room temperature) for 12 hours, filter, take the supernatant as the extract I, collect the residue, and count it as the residue I; then extract the residue I with 55L of 70% ethanol, the extraction conditions are the same as above, and the extracted Liquid II, Residue II. The extract I and the extract II were combined to obtain the total extract.

[0046] The total extract was distilled under reduced pressure at a water temperature of 40°C to obtain 450.6g primary extract.

[0047] 2. Dissolve 450.1g primary extract in water...

Embodiment 2

[0075] Example 2, Antitumor Activity Detection of Compound 1 and Compound 2

[0076] Lung cancer cells (NCI-H460 cells) were purchased from the Cell Bank of the Chinese Academy of Sciences, the catalog number is TCHu 38, breast cancer cells (SF-268 cells) were purchased from the Cell Bank of the Chinese Academy of Sciences, the catalog number is TCHu 49, breast cancer cells (MCF -7 cells) were purchased from the Cell Bank of the Chinese Academy of Sciences, the product catalog number is TCHu 74, and human liver cancer cells (human HepG2 cells) were purchased from the Cell Bank of the Chinese Academy of Sciences, and the product catalog number was TCHu 72.

[0077]Inhibition experiments of compounds on tumor cells were carried out using the method described in the literature (Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytoxicity assays. Journal of Immunological Methods, 65, 55-63). MTT method (Mosmann, 1983) d...

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Abstract

The invention discloses steroid saponin compounds and a preparation method and application thereof. The preparation method comprises the following steps of: extracting tribulus terrestris linn by using an extraction agent to obtain extract; performing high performance liquid chromatography on the extract to obtain the compounds shown as a formula I and / or a formula II in the claim 1, wherein the high performance liquid chromatography comprises the following steps of: taking mixed solution consisting of acetonitrile and water in a volume ratio of 35 to 65 as a mobile phase, wherein the flow rate of the mobile phase is 4ml / minute; collecting an elution peak with retention time of 9.798 to 10.798 minutes to obtain the compound shown as the formula I; and collecting the elution peak with the retention time of 13.423 to 14.423 minutes to obtain the compound shown as the formula II. An experiment shows that: the two steroid saponin compounds have good effect of suppressing tumors, and can suppress a plurality of tumors; and the method for preparing the steroid saponin compounds has the advantages of simple operation, low cost and high yield. Therefore, the compounds have wide application prospect in preventing and / or treating the tumors.

Description

technical field [0001] The invention relates to a steroidal saponin compound, a preparation method and application thereof. Background technique [0002] According to the records in "National Compilation of Traditional Chinese Medicine. Volume 1", "Practical Chinese Herbal Medicine Color Picture Book" Volume 2, and "Commonly Used Chinese Medicine Identification Encyclopedia", Tribulus terrestris, also known as Tribulus terrestris, is the dried fruit of Tribulus terrestris Linn., a plant of the family Tribulus. At present, there are many reports on the pharmacological effects of total saponins of Tribulus terrestris, but no pharmacological research on its single component. According to literature research, the total saponins of Tribulus terrestris mainly have the following pharmacological activities (Chinese Wild Plant Resources Vol.22No.4Aug.2003): [0003] 1. The effect on the cardiovascular system [0004] The total saponins of Tribulus terrestris have protective effects...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00C07J75/00A61K31/7048A61P35/00
Inventor 蒋宇扬王珏祖旭宇谭春燕刘红霞
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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