Steroid saponin compounds and preparation method and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for steroidal saponins and compounds is applied in the fields of steroidal saponin compounds and their preparation and application, and achieves the effects of low cost, high yield and good effect
Inactive Publication Date: 2010-11-03
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
View PDF0 Cites 4 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Recent studies have shown that the above-mentioned effects of saponins may be related to their ability to increase the content of sex hormones in the body
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0042] Embodiment 1, the preparation of steroidal saponin compound
[0043] Tribulus terrestris was purchased from Shenzhen Research Center of Traditional Chinese Medicine, Guangdong Province, the product catalog number is TT2002061211.
[0044] 1. Preparation
[0045] 1. Take 2.5kg of Tribulus terrestris and pulverize it, and extract it with 55L of 70% ethanol (ethanol:water=70:30, volume ratio). The extraction steps are as follows: cold soak at 25°C (room temperature) for 12 hours, filter, take the supernatant as the extract I, collect the residue, and count it as the residue I; then extract the residue I with 55L of 70% ethanol, the extraction conditions are the same as above, and the extracted Liquid II, Residue II. The extract I and the extract II were combined to obtain the total extract.
[0046] The total extract was distilled under reduced pressure at a water temperature of 40°C to obtain 450.6g primary extract.
[0047] 2. Dissolve 450.1g primary extract in water...
Embodiment 2
[0075] Example 2, Antitumor Activity Detection of Compound 1 and Compound 2
[0076] Lung cancer cells (NCI-H460 cells) were purchased from the Cell Bank of the Chinese Academy of Sciences, the catalog number is TCHu 38, breast cancer cells (SF-268 cells) were purchased from the Cell Bank of the Chinese Academy of Sciences, the catalog number is TCHu 49, breast cancer cells (MCF -7 cells) were purchased from the Cell Bank of the Chinese Academy of Sciences, the product catalog number is TCHu 74, and human liver cancer cells (human HepG2 cells) were purchased from the Cell Bank of the Chinese Academy of Sciences, and the product catalog number was TCHu 72.
[0077]Inhibition experiments of compounds on tumor cells were carried out using the method described in the literature (Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytoxicity assays. Journal of Immunological Methods, 65, 55-63). MTT method (Mosmann, 1983) d...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses steroid saponin compounds and a preparation method and application thereof. The preparation method comprises the following steps of: extracting tribulus terrestris linn by using an extraction agent to obtain extract; performing high performance liquid chromatography on the extract to obtain the compounds shown as a formula I and/or a formula II in the claim 1, wherein the high performance liquid chromatography comprises the following steps of: taking mixed solution consisting of acetonitrile and water in a volume ratio of 35 to 65 as a mobile phase, wherein the flow rate of the mobile phase is 4ml/minute; collecting an elution peak with retention time of 9.798 to 10.798 minutes to obtain the compound shown as the formula I; and collecting the elution peak with the retention time of 13.423 to 14.423 minutes to obtain the compound shown as the formula II. An experiment shows that: the two steroid saponin compounds have good effect of suppressing tumors, and can suppress a plurality of tumors; and the method for preparing the steroid saponin compounds has the advantages of simple operation, low cost and high yield. Therefore, the compounds have wide application prospect in preventing and/or treating the tumors.
Description
technical field [0001] The invention relates to a steroidal saponin compound, a preparation method and application thereof. Background technique [0002] According to the records in "National Compilation of Traditional Chinese Medicine. Volume 1", "Practical Chinese Herbal Medicine Color Picture Book" Volume 2, and "Commonly Used Chinese Medicine Identification Encyclopedia", Tribulus terrestris, also known as Tribulus terrestris, is the dried fruit of Tribulus terrestris Linn., a plant of the family Tribulus. At present, there are many reports on the pharmacological effects of total saponins of Tribulus terrestris, but no pharmacological research on its single component. According to literature research, the total saponins of Tribulus terrestris mainly have the following pharmacological activities (Chinese Wild Plant Resources Vol.22No.4Aug.2003): [0003] 1. The effect on the cardiovascular system [0004] The total saponins of Tribulus terrestris have protective effects...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more