Pressure-electricity co-stimulation cell culture device

A technology for cell culture and cell stimulation, which is applied in the field of cell engineering and tissue engineering, can solve problems such as inability to simulate cells in vivo, and achieve the effects of improving cell growth conditions, cell activity, and stability

Inactive Publication Date: 2010-11-24
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the technical problem that the existing methods cannot simulate the physical microenvironment in which the cells in the body are under pressure and electrical stimulation simultaneously, and propose a pressure-electricity combined stimulation cell culture device, which uses pressure stimulation and electrical stimulation to jointly load, Place cells under the combined action of pressure stimulation and electrical stimulation to simulate the living environment of cells in vivo, promote cell growth and differentiation, and construct functional cells or tissues

Method used

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  • Pressure-electricity co-stimulation cell culture device
  • Pressure-electricity co-stimulation cell culture device
  • Pressure-electricity co-stimulation cell culture device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The liquid type I collagen is mixed with the cell culture medium, the density of the bone marrow mesenchymal stem cell suspension is adjusted to 5×106 cells / ml, and then the bone marrow mesenchymal stem cells are mixed with the above mixture. Add the mixed solution into the round hole of the cell culture plate, and place it in the cell culture incubator for compound culture. The cell-material composite culture 404 is formed and taken out for use.

[0051] The cell culture chamber fixing device 2, the transmission device 3, the cell culture chamber 4, etc. are sterilized and then assembled. First put a circular platinum electrode piece into the bottom of the lower cavity 405 , and fix the other platinum electrode on the top of the pressure head 403 of the upper cavity 402 with glue. The connecting rod 401 of the upper cavity 402 is fixed on the fastening ring 303 of the transmission plate 302 , so that the upper cavity 402 is fixed on the transmission plate 302 and conn...

Embodiment 2

[0055] Under aseptic conditions, the radiation-sterilized cross-linked gelatin / chitosan scaffold material was taken out and placed in a cell culture plate. Monolayer cultured osteoblasts were digested with trypsin solution to make cell suspension, the concentration of cell suspension was 1.0×10 6 Cells / ml, add the cell suspension into the biological material through the injection needle, add a large amount of culture medium after the cells attach to the material, and culture statically for 2 days.

[0056] The cell culture chamber fixing device 2, the transmission device 3, the cell culture chamber 4, etc. are sterilized and then assembled. First put a round stainless steel electrode piece into the bottom of the lower chamber 405, and fix the other stainless steel electrode on the top of the pressure head 403 of the upper chamber 402 with glue. The connecting rod 401 of the upper cavity 402 is fixed on the fastening ring 303 of the transmission plate 302 , so that the upper c...

Embodiment 3

[0060] Under aseptic conditions, the radiation-sterilized polylactic acid (PLLA) / β-tricalcium phosphate (β-TCP) porous scaffold material is taken out and placed in a cell culture plate. Monolayer cultured chondrocytes were digested with trypsin solution to make cell suspension, the concentration of cell suspension was 1.0×10 6 Cells / ml, add the cell suspension into the PLLA / β-TCP biomaterial through the injection needle, add a large amount of culture medium after the cells attach to the material, and culture statically for 2 days.

[0061] The cell culture chamber fixing device 2, the transmission device 3, the cell culture chamber 4, etc. are sterilized and then assembled. First put a circular platinum electrode piece into the bottom of the lower cavity 405 , and fix the other platinum electrode on the top of the pressure head 403 of the upper cavity 402 with glue. The connecting rod 401 of the upper cavity 402 is fixed on the fastening ring 303 of the transmission plate 302...

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Abstract

The invention discloses a pressure-electricity co-stimulation cell culture device. The device comprises a cell incubator, a cell culture cavity fixing device, a cell culture cavity, a transmission device, a stepper motor and a signal generator, wherein the incubator is provided with a pore canal communicated with the outside; the cell culture cavity is fixed on the cell culture cavity fixing device; the cell culture cavity comprises a connecting rod, an upper cavity, a pressure head, a cell-material composite culture and a lower cavity; the upper cavity is fixed on a transmission plate of thetransmission device and is connected with the stepper motor through a sliding screw rod; the lower cavity is fixed in a circular groove of a base of the cell culture cavity fixing device; the cell-material composite culture is arranged in the lower cavity; and the signal generator is connected with a metal electrode in the cell culture cavity. The device has the advantages of overcoming the defect that a physical microenvironment for in vivo cells under which pressure stimulation and electricity stimulation coexist cannot be simulated by the conventional method, establishing a pressure-electricity co-stimulation cell culture method and promoting the development of cells, a tissue culture method and a relevant tissue engineering reactor.

Description

technical field [0001] The invention relates to a pressure-electricity combined stimulation cell culture device, which belongs to the technical fields of cell engineering and tissue engineering. Background technique [0002] In tissue engineering, the in vitro functional culture of cells and tissues has become the core of tissue engineering and the necessary technical basis for the formation of tissue engineering industry. Providing an in vitro growth environment similar to the living conditions of cells in vivo is crucial for the three-dimensional culture and functionalization of cells and tissues, in which physical factors such as mechanics and electricity play a role that cannot be ignored. [0003] The mechanical environment has an important impact on life activities at all levels of organs, tissues, cells and even sub-cells. Mechanical force can activate a variety of mechanically sensitive cells, such as endothelial cells, smooth muscle cells, osteoblasts, and osteocla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/42
CPCC12M35/02
Inventor 李萍樊瑜波宋崴冯利敏李钰刘李珍
Owner BEIHANG UNIV
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