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Corynebacterium humireducens and application thereof

A Corynebacterium, humus technology, applied in bacteria, biochemical fuel cells, microorganisms and other directions, can solve problems such as unfavorable microbial growth, strains with humus reduction and electricity-generating activity have not been found, and achieve high-efficiency humus reduction capacity and electricity-producing activity, The effect of stable electricity production and wide utilization spectrum

Inactive Publication Date: 2012-07-04
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alkaline conditions help to improve the efficiency of electricity production, but the alkaline environment is not conducive to the growth of most microorganisms, so this patent aims to develop alkaline humus reducing and electricity-producing microorganisms
After literature and patent searches, no reports have been found on strains with humus reducing and electrogenic activity under alkaline conditions

Method used

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  • Corynebacterium humireducens and application thereof
  • Corynebacterium humireducens and application thereof
  • Corynebacterium humireducens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Separation and purification of Corynebacterium humicum reducing of the present invention

[0035] 1) Take 5 mL of anolyte from the sludge microbial fuel cell with pH of 10 and inoculate it into 100 mL of liquid separation medium.

[0036] The structure of the sludge microbial fuel cell has been described in detail in the patent application No. 200910041235.4.

[0037] The composition of the liquid separation medium is: each liter of the liquid separation medium contains 1.0g glucose (electron donor), 16g AQDS (electron acceptor), 2.5g NaHCO 3 , 0.25gNH 4Cl, 0.678g NaH 2 PO 4 ·2H 2 O, 0.1 g KCl, 10.0 mL vitamin solution, 10.0 mL trace element solution, pH 10. Among them, the vitamin solution contains 2.0mg biotin, 2.0mg folic acid, 10.0mg vitamin B per liter of deionized water 6 , 5.0mg thiamine, 5.0mg riboflavin, 5.0mg niacin, 5.0mg calcium pantothenate, 0.1mg vitamin B 12 , 5.0mg p-aminobenzoic acid, 5.0mg lipoic acid; trace element solution is 1.5g n...

Embodiment 2

[0042] Example 2: Physiological and biochemical properties of Corynebacterium humireducens

[0043] The strains are non-fermentative facultative anaerobic bacteria, resistant to salt and alkali. The growth temperature range is 25-40°C, and the growth cannot be performed above 45°C and below 10°C; the growth pH range is 7.0-11.0 (optimum: pH9.0); the NaCl% required for growth is 0-12% (>13% cannot be grown) growth). According to the physiological and biochemical characteristics, the bacteria was identified as Corynebacteria.

[0044] Table 1 Physiological and biochemical characteristics of inventive strains

[0045]

[0046]

[0047] + means positive reaction; - means negative reaction

Embodiment 3

[0048] Example 3: Molecular taxonomic status of strains (Corynebacterium humireducens)

[0049] as attached figure 2 As shown, the 6 strains with the closest homology to the invented strain belong to Corynebacterium, namely: C. marinum (similarity 96.9%, 1393 bp), C. testudinoris (96.8%, 1395 bp), C. halotolerans (96.4% , 1392bp), C.felinum (96.0%, 1383bp), C.singulare (96.0%, 1297bp) and C.freiburgense (95.9%, 1395bp). According to the phylogenetic theory, the similarity between strains can only be considered to be the same strain if the similarity is greater than 97%, and the similarity between the target strain and its closest strain C. marinum is only 96.9%, so the strain is defined as a new species.

[0050] Combined with the above-mentioned physiological and biochemical characteristics and 16S rRNA sequence comparison results, the present invention belongs to the genus Corynebacterium, which is a new species of this genus, and is named as Corynebacterium humireducens. ...

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Abstract

The invention discloses corynebacterium humireducens and an application thereof. The corynebacterium humireducens is obtained by separating and purifying anolyte of a sludge microbe fuel cell, and is preserved in the ordinary microbe centre of the China Microbe Culture Preservation Management Committee on April 14, 2008 with the preservation number of CGMCC No.2452. The corynebacterium humireducens has wide utilization spectrum of electron donor under the anaerobic condition, has efficient humus reduction capability and electrogenesis capability under the alkaline condition, can be widely applied to in-situ microbe restoration of organic pollutants, microbe fuel cell generating technology or recycling processing of high-concentration organic wastes.

Description

technical field [0001] The invention belongs to the technical field of environment and new energy, and in particular relates to a Corynebacterium humicus-reducing bacterium with electricity-generating and humus-respiration activity and application thereof. technical background [0002] Humus respiration is a new type of microbial energy metabolism that has received much attention in recent years. Humic respiration, also known as quinone respiration, means that microorganisms use humic as the terminal electron acceptor, couple the humic to reduce through oxidation electron donor, and store the energy of life activities from this process. Microorganisms capable of humic respiration are called humic reducing bacteria. At present, the environmental importance of humic respiration has been widely accepted and recognized: humic respiration is not only coupled with the natural cycle process of carbon, nitrogen, phosphorus and other elements, but also significantly affects the redu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20H01M8/16C12R1/15
CPCY02E60/527Y02E60/50
Inventor 周顺桂武春媛王跃强李芳柏袁勇
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI