Unlock instant, AI-driven research and patent intelligence for your innovation.

A biosensor device and a method of sequencing biological particles

A biosensor, sensor technology, used in biochemical equipment and methods, determination/inspection of microorganisms, etc.

Inactive Publication Date: 2010-11-24
NXP BV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Sanger method is also limited by the liquid-gel electrophoresis method performed in the context of the traditional Sanger method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A biosensor device and a method of sequencing biological particles
  • A biosensor device and a method of sequencing biological particles
  • A biosensor device and a method of sequencing biological particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0075] The descriptions in the figures are schematic. In different figures, similar or identical elements are provided with the same reference signs.

[0076] In the following, refer to figure 1 , illustrating a biosensor device 100 for sequencing a DNA molecule 102 according to an exemplary embodiment of the present invention.

[0077] The biosensor device 100 includes a silicon substrate 104 . A plurality of sensor active regions 106 are provided on the surface of the silicon substrate 104 . On each sensor active region 106 , a primer molecule 108 , which is an oligonucleotide complementary to the end of the DNA sequence 102 , is immobilized. The primer 108 has a sequence complementary to the end of the sequence of the bioparticle 102 . Thus, the upper portion of the DNA 102 remains exposed to the fluid environment 130 in which the nucleotide bases 110 (A, T, C, and G) and DNA polymerase 112 as a replicase are present.

[0078] can be made by figure 1 The electrical si...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
Login to View More

Abstract

A biosensor device (100) for sequencing biological particles (102), the biosensor device (100) comprising at least one substrate (104), a plurality of sensor active regions (106) provided on each of the at least one substrate (104) and each comprising a primer (108) having a sequence being complementary to a part of a sequence of the biological particles (102) and enabling generation of fragments having a sequence being inverse to a part ofthe sequence of the biological particles (102) at the primer(108), and a determination unit (114) adapted for individually determining a size of the fragments generated at the primer (108) of each of the plurality of sensor active regions (106), the fragment replication being terminated in the presence of replication terminating sequence units (116 to 119).

Description

technical field [0001] The present invention relates to biosensor devices. [0002] Furthermore, the present invention relates to methods for sequencing biological particles. Background technique [0003] A biosensor may mean a device for detecting an analyte that combines a biological component with a physicochemical or physical detector component. [0004] For example, biosensors can be based on the following phenomena: for example, when the antibody-binding fragments of antibodies or DNA single-strand sequences as capture molecules match the corresponding sequences or structures of target molecules, the capture molecules immobilized on the surface of the biosensor can selectively interact with Hybridization of target molecules in a fluid sample. When such a hybridization or sensor event occurs on the sensor surface, this can change the surface electrical properties, which can be detected as a sensor event. [0005] DNA sequencing is an important application of biochemi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6874C12Q2565/607C12Q2565/601C12Q2565/537C12Q2535/101
Inventor 巴勃罗·加西亚特洛
Owner NXP BV