Transformant for blocking chicken myostatin effect by oral immunity and application thereof

A technology for transformants and immune preparations, applied in the field of bioengineering, can solve the problems of difficult realization, waste, and labor of protein preparations, and achieve the effects of enhancing the immune response of the body, improving the immune effect, and increasing the safety.

Inactive Publication Date: 2012-02-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Protein purification steps greatly increase the cost and difficulty of production, making it difficult to achieve large-scale production of target protein preparations
At the same time, the injection immunization procedure is also cumbersome, which not only consumes a lot of manpower, but also causes greater stress and damage to animals.

Method used

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  • Transformant for blocking chicken myostatin effect by oral immunity and application thereof
  • Transformant for blocking chicken myostatin effect by oral immunity and application thereof
  • Transformant for blocking chicken myostatin effect by oral immunity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] GM17 liquid medium: tryptone 2%; yeast extract 0.5%; Vc 0.05%; beef extract 0.5%; sodium acetate 0.15%; sodium chloride 0.4%; MgSO 4 .7H 2 O 0.25%; Glucose 0.5%, adjusted to pH 6.8. GM17 solid medium was supplemented with 1.6% agar on the basis of the above medium.

[0021] Lactococcus lactis NZ9800 and vector pNZ8112 are both commercial products from NIZO food research in the Netherlands.

[0022] 1. Construction and cloning of the target gene

[0023] (1) Acquisition of CTB gene mature region:

[0024] Upstream primer CTB P1 (SEQ ID NO.1):

[0025] 5'-GG ACACCTCAAAATATTACTG -3'

[0026] The primer structure is: protected base (GG)-Xba I site (sequence in the box)-CTB 5' end partial sequence (underlined sequence)

[0027] Downstream primer CTB P2 (SEQ ID NO.2):

[0028] 5'-TG ATTTGCCATACTAATTG -3'

[0029] Primer structure: protection base (TG)-EcoR I site (sequence in box)-CTB 3' end partial sequence (underline).

[0030] Using the above primers and us...

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Abstract

The invention discloses a transformant, which comprises a recipient bacterium and a recombinant vector transformed into the recipient bacterium, wherein the recipient bacterium is Lactococcus lactis NZ9800; an original vector of the recombinant vector is pNZ8112; and a foreign target gene of the recombinant vector comprises a chicken myostatin gene, a cholera toxin B subunit gene and a cell wall anchor element M6 gene. The transformant can enter the intestinal tract of chicken in an oral administration mode, and has the advantages of reducing the toxicity caused by directly contacting cyclic system and improving the safety of vaccine compared with injection immunity. An antigen gene / immune-enhancing factor is selected for combined immunity, so the immune response of organisms is enhanced and the immune effect is improved.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a transformant capable of oral immune blocking effect of chicken myostatin and application thereof. Background technique [0002] Poultry products are one of the main animal foods for humans. With the continuous improvement of people's living standards, the market demand for poultry products is increasing. my country is a country with many poultry breeds, and the resources of local chicken breeds are very rich. Compared with some "special poultry breeds" bred abroad, these breeds in my country have high adaptability to the surrounding environment, resistance to extensive management, strong disease resistance, The quality of meat and eggs is good. However, due to the slow growth of local chicken breeds, low feed utilization rate, low egg production, and the introduction of foreign high-performance breeds, many local chicken breeds have gradually decreased or even become endangered o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21A61K39/00A61K35/74A61P43/00C12R1/01
Inventor 牛冬
Owner ZHEJIANG UNIV
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