Method for producing organic acid by fermenting abandoned thallus with monosodium glutamate
A technology of waste bacteria and organic acids, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of consuming cultivation time, material energy, consumption, etc., to reduce production costs, reduce pollution, and control carbon The effect of emissions
Examples
Embodiment 1
[0016] Collect the waste cells of Corynebacterium glutamicum ATCC 10128 used in monosodium glutamate industry to ferment L-glutamic acid, and finally obtain a biomass of 10g-dry cell×L -1 . (OD 600nm =0.25g-dry cell×L -1 ).
[0017] Fully suspend the sludge with an equal volume of anaerobic medium at the end of aerobic conditions, transfer it to an anaerobic fermentation tank, and then add CO 2 The gas flows into the anaerobic fermentation medium of the fermenter at an aeration rate of 0.2v / vm, the stirring speed is 200rpm, the temperature is controlled at 30°C, and the anaerobic fermentation is carried out for 24 hours.
[0018] Anaerobic fermentation medium: glucose 40g / L, K 2 HPO 4 ·3H 2 O 0.5g / L, KH 2 PO 4 0.5g / L, MgSO 4 ·7H 2 O0.5g / L, FeSO 4 ·7H 2 O 6mg / L, MnSO 4 ·H 2 O 6mg / L, biotin 0.2mg / L, vitamin B1 200μg / L, NaHCO 3 42g / L. Initial pH 7.0.
[0019] As a result of fermentation, the main conversion products are lactic acid, acetic acid and succinic a...
Embodiment 2
[0021] Collect the waste cells of Corynebacterium glutamicum ATCC 10128 used in monosodium glutamate industry to ferment L-glutamic acid, and finally obtain a biomass of 10g-dry cell×L -1 . (OD 600nm =0.25g-dry cell×L -1 ).
[0022] Fully suspend the sludge with an equal volume of anaerobic medium at the end of aerobic conditions, transfer it to an anaerobic fermentation tank, and then add CO 2 The gas flows into the anaerobic fermentation medium of the fermenter at an aeration rate of 0.2v / vm, the stirring speed is 200rpm, the temperature is controlled at 30°C, and the anaerobic fermentation is carried out for 32 hours.
[0023] Anaerobic fermentation medium: glucose 80g / L, K 2 HPO 4 ·3H 2 O 0.5g / L, KH 2 PO 4 0.5g / L, MgSO 4 ·7H 2 O0.5g / L, FeSO 4 ·7H 2 O 6mg / L, MnSO 4 ·H 2O 6mg / L, biotin 0.2mg / L, vitamin B1 200μg / L, NaHCO 3 42g / L. Initial pH 7.0.
[0024] As a result of fermentation, the main conversion products are lactic acid, acetic acid and succinic ac...
Embodiment 3
[0026] Collect the waste cells of Corynebacterium glutamicum ATCC 10129 used in MSG industry to ferment L-glutamic acid, and finally obtain a biomass of 10g-dry cell×L -1 . (OD 600nm =0.25g-dry cell×L -1 ).
[0027] Suspend the treated sludge with an equal volume of anaerobic medium at the end of aerobic conditions, transfer it to an anaerobic fermentation tank, and then add CO 2 The gas flows into the anaerobic fermentation medium in the fermenter at an aeration rate of 0.2v / vm, the stirring speed is 200rpm, the temperature is controlled at 30°C, and the anaerobic fermentation is carried out for 28 hours.
[0028] Anaerobic fermentation medium: glucose 40g / L, K 2 HPO 4 ·3H 2 O 0.5g / L, KH 2 PO 4 0.5g / L, MgSO 4 ·7H 2 O0.5g / L, FeSO 4 ·7H 2 O 6mg / L, MnSO 4 ·H 2 O 6mg / L, biotin 0.2mg / L, vitamin B1 200μg / L, NaHCO 3 42g / L. Initial pH 7.0.
[0029] As a result of fermentation, the main conversion products are lactic acid, acetic acid and succinic acid, the yields...
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Description
Claims
Application Information
- IPC
- C12P7/46; C12P7/54; C12P7/56; C12R1/15
- CPC
- Y02P20/141
- Inventors
- 严明; 郝宁