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Porous chitosan scaffold, and neural stem cell porous chitosan scaffold and application thereof

A technology of porous chitosan and neural stem cells, applied in medical science, prosthesis, etc., can solve the problems of lack of three-dimensional space material basis, unfavorable NSCs survival and migration, unfavorable NSCs, etc., to promote the improvement and inhibition of cognitive function. The formation of glial scars, the effect of improving learning

Inactive Publication Date: 2011-02-23
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Simple cell transplantation is not conducive to the survival and migration of NSCs due to the lack of necessary three-dimensional space material basis, and it is also not conducive to the application of intervention factors to promote the differentiation of NSCs into neurons

Method used

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  • Porous chitosan scaffold, and neural stem cell porous chitosan scaffold and application thereof
  • Porous chitosan scaffold, and neural stem cell porous chitosan scaffold and application thereof
  • Porous chitosan scaffold, and neural stem cell porous chitosan scaffold and application thereof

Examples

Experimental program
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Embodiment Construction

[0014] Part I: In Vitro Experiments

[0015] Preparation of Chitosan Scaffold and Its Biocompatibility with Neural Stem Cells

[0016] 1 Experimental materials

[0017] 1.1 Experimental animals

[0018] 15d pregnant Sprague-Dawley (SD) rats, clean grade, provided by the Experimental Animal Center of Nantong University, production license number: SCXK (Su) 2002-0019.

[0019] 1.2 Experimental Instruments

[0020] ① Low-temperature freeze-drying apparatus (Lyopro3000, France Jouan company);

[0021] ② CO 2 Incubator (French Jouan company);

[0022] ③Ultra-clean workbench (SW-CJ-1F, Suzhou Antai Air Technology Co., Ltd.);

[0023] ④Low temperature refrigerated centrifuge (Beckman Company, USA);

[0024] ⑤Leica CM1900 cryostat cryostat (Germany);

[0025] ⑥ Leica DMR upright fluorescence microscope (Germany);

[0026] ⑦ Leica DMIRB inverted fluorescence microscope (Germany);

[0027] ⑧NUAIRE ultra-low temperature refrigerator (-85℃, USA);

[0028] ⑨ Jetta 801 series mor...

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Abstract

The invention discloses a porous chitosan scaffold, and a neural stem cell porous chitosan scaffold and application thereof. A method for preparing the porous chitosan scaffold comprises the following steps of: dissolving chitosan in acetic acid to form 2 percent solution, adding the solution into pores of a cell culture plate, freeze-drying, adding NaOH into the pores of the cell culture plate for hydration, and freeze-drying twice at the temperature of -60 DEG C to obtain the porous chitosan scaffold. A method for preparing the neural stem cell porous chitosan scaffold comprises the following steps of: inoculating neural stem cell clone spheres to the sterilized porous chitosan scaffold in the culture spores, and adding a DMEM / F12 serum-free culture medium or an NGF-containing DMEM / F12 serum-free culture medium for culture to obtain the neural stem cell porous chitosan scaffold. The chitosan porous scaffold prepared by a low-temperature freeze-drying method has uniform pores, the porosity of 90 percent, the average pore size of 50 to 350mu m and high biocompatibility with neural stem cells (NSCs). Traumatic brain injury (TBI) is treated by transplanting the NSCs using chitoson as a carrier, and cognitive functions after injury, such as learning, memorizing and the like can be obviously improved.

Description

technical field [0001] The present invention relates to a tissue engineering product and its application. Background technique [0002] The regeneration of the central nervous system (central nervous system, CNS) after injury has always been a difficult problem for humans, although Reynolds and Richards first isolated neural stem cells (neural stem cells, NSCs) from the striatum and hippocampus of adult mice in 1992. ), and since then it has been discovered that NSCs also exist in other regions such as the cerebral cortex, olfactory bulb, septum, and spinal cord in the CNS of adult mammals, thus breaking the traditional concept that the CNS cannot regenerate after adulthood, but endogenous NSCs can respond to the CNS. The injury has a limited ability to generate new functional neurons. NSCs can expand and differentiate into neurons, astrocytes, and oligodendrocytes in vitro. With the maturity of embryonic and adult neural stem cell isolation and culture techniques, transpl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/38
Inventor 金国华衣昕田美玲秦建兵毛伟峰高宜录
Owner NANTONG UNIVERSITY
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