Method for producing avermectin by utilizing fermentation wastewater and carbohydrate supplement process
A technology of abamectin and fermented wastewater, applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of high investment and treatment cost of wastewater treatment equipment, restricting the healthy development of abamectin production enterprises, Increase the production cost of abamectin and other issues to achieve the effect of increasing the fermentation titer, reducing the water used for sugar preparation, and reducing the processing cost
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Embodiment 1
[0030] 1) Inoculate the cultured Streptomyces avermitilis seeds into a 50L fermentor equipped with a fermentation medium for fermentation, the fermentor culture temperature is 27.5 ° C, and the rotating speed and air volume are appropriately adjusted to ensure that the dissolved oxygen is more than 30%, and the The charging factor is 70%;
[0031] 2) fermentation initial stage (that is, fermentation 100 hours), in above-mentioned fermentation culture liquid, add the abamectin fermentation treatment waste water after sterilization, the addition of abamectin fermentation treatment waste water is equal to the evaporation of fermentation culture liquid in the fermentor Abamectin fermentation treatment wastewater is calculated by adding 3% to 5% of the fermentation broth per day, i.e. 1 to 1.5 liters per day, that is, 4 to 6 liters of abamectin are added in 100 hours for fermentation treatment. waste water;
[0032] 3) after 100 hours of fermentation, carry out sugar supplementati...
Embodiment 2
[0036] 1) Inoculate the cultivated Streptomyces avermitilis seeds into a 50L fermenter equipped with a fermentation medium for fermentation, the fermentor culture temperature is 28°C, and the rotating speed and air volume are appropriately adjusted to ensure that the dissolved oxygen is more than 30%, and the The charging factor is 70%;
[0037]2) in the early stage of fermentation (i.e. fermentation 70 hours), add the sterilized abamectin fermentation treatment waste water in the above-mentioned fermentation culture liquid, the addition amount of abamectin fermentation treatment waste water is equal to the evaporation of the fermentation culture liquid in the fermenter Amount, abamectin fermented waste water is added one-time evaporation, after 70 hours, add 3 to 4.5 liters of abamectin fermented waste water;
[0038] 3) After 120 hours of fermentation, the sugar supplement operation is carried out, that is, the glucose solution prepared by fermenting and treating wastewater ...
Embodiment 3
[0042] 1) Inoculate the cultured Streptomyces avermitilis seeds into a 50L fermenter equipped with fermentation broth for fermentation. The culture temperature of the fermenter is 28.5°C, and the rotating speed and air volume are properly adjusted to ensure more than 30% of dissolved oxygen. The charging factor is 70%;
[0043] 2) During fermentation, add the sterilized Abamectin fermentation treatment waste water in the above-mentioned fermentation culture liquid, the addition amount of Abamectin fermentation treatment waste water is equal to the evaporation of fermentation culture liquid in the fermenter;
[0044] 3) After 140 hours of fermentation, carry out the sugar supplement operation, that is, add the glucose solution prepared by fermenting and treating wastewater with abamectin in the above-mentioned fermentation culture medium, and the sugar supplement amount is 10g / day and stop the sugar supplement after 260 hours of fermentation;
[0045] 4) Fermentation ends after...
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