Method for preparing hovenia polysaccharides
A technology of jujube and polysaccharide, which is applied in the field of plant active ingredient extraction to achieve the effects of energy saving, method safety and no pollution to the environment
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Embodiment 1
[0017] Wash the jujube and dry it in the shade, crush it into a coarse powder, pass through a 40-mesh sieve, weigh 500g and place it in an ultrasonic extractor, add 1L of distilled water and stir well, soak for 12 hours, add water until the ratio of solid to liquid is 1:10, Under the ultrasonic power of 200W, ultrasonically extract for 1 hour, repeatedly extract 4 times, filter, combine the extracts, concentrate to 500ml, add acid to adjust the pH to 5, and prepare the enzyme according to the weight ratio of amylase: protease=5:1 Preparation, add 1.25g, react at 45°C for 6 hours, pass the enzymolysis solution through the HZ802 macroporous adsorption resin column, add ethanol to the permeate solution to make the ethanol concentration 80%, let it stand for 3 hours, and then centrifuge for 15 minutes. The jujube polysaccharide was obtained and freeze-dried to obtain 52 g of finished product with a content of 64.8%.
Embodiment 2
[0019] Wash the jujube and dry it in the shade, crush it into a coarse powder, pass through a 60-mesh sieve, weigh 500g and place it in an ultrasonic extractor, add 1.5L of distilled water and mix well, soak for 18 hours, add water until the ratio of solid to liquid is 1:20 , under the ultrasonic power of 250W, ultrasonically extract 1.5 hours, repeatedly extract 2 times, filter, combine extract, extract is concentrated to 500ml, add acid to adjust pH to 6, by the weight of amylase: protease=5: 1 Prepare an enzyme preparation, add 2.5g, react at 40°C for 5 hours, pass the enzymolysis solution through a D101 macroporous adsorption resin column, add ethanol to the permeate to make the ethanol concentration 80%, let it stand for 4 hours, and then Centrifuge for 15 minutes to obtain jujube polysaccharide, freeze-dry to obtain 50 g of finished product with a content of 62.4%.
Embodiment 3
[0021] Wash the jujube and dry it in the shade, crush it into a coarse powder, pass through a 60-mesh sieve, weigh 1000g and place it in an ultrasonic extractor, add 2.2L of distilled water and mix well, soak for 14 hours, add water until the ratio of solid to liquid is 1:14 , under the ultrasonic power of 400W, ultrasonically extract for 2 hours, repeatedly extract 4 times, filter, combine the extracts, concentrate the extracts to 1L, add acid to adjust the pH to 5.5, and prepare the amylase:protease=5:1 by weight Prepare an enzyme preparation, add 4.0g, react at 45°C for 7 hours, pass the enzymolysis through the HZ802 macroporous adsorption resin column, add ethanol to the permeate to make the ethanol concentration 75%, let it stand for 4 hours, and then centrifuge In 15 minutes, the jujube polysaccharide was obtained, which was freeze-dried to obtain 106 g of finished product with a content of 64.4%.
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