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Application of BZ6-1 bacterial strain in preparing drugs for treating plant peanut bacterial wilt

A technology for peanut bacterial wilt disease and strains, which is applied in the field of plant pesticides and can solve the problems that no relevant reports have been reported in the screening of endogenous antagonistic bacteria against peanut bacterial wilt disease.

Active Publication Date: 2011-04-20
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chen Min [Chen Min, Xu Lijun, Wu Binjuan, et al. Preliminary identification and culture optimization of endophytic antagonistic strain HE-1 against cucumber bacterial wilt [J]. Science and Technology Bulletin, 2008, 24(4): 489-493.] et al. isolated an endophytic antagonistic strain HE-1 from cucumber, which has a good antibacterial effect on cucumber R. solanacearum, but there is no relevant report on the screening of endophytic antagonistic bacteria against peanut R. solanacearum

Method used

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  • Application of BZ6-1 bacterial strain in preparing drugs for treating plant peanut bacterial wilt
  • Application of BZ6-1 bacterial strain in preparing drugs for treating plant peanut bacterial wilt
  • Application of BZ6-1 bacterial strain in preparing drugs for treating plant peanut bacterial wilt

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Experimental program
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Embodiment 1

[0011] 1 Materials and methods

[0012] 1.1 Strains

[0013] Healthy peanut plants were collected from the long-term peanut continuous cropping test area of ​​the Red Soil Ecological Experiment Station of the Chinese Academy of Sciences, and Ralstonia solanacearum provided by the Plant Pathology Laboratory of the School of Plant Protection, Nanjing Agricultural University was used as an indicator bacteria for screening antagonistic bacteria. Rice blast fungus (Magnaprothe grisea), wheat sheath blight fungus (Rhizoctonia cerealis), wheat scab fungus (Fusarium graminearum), wheat root rot fungus (Helminthosporium sativum) were provided by the Plant Pathology Laboratory of the College of Horticulture and Plant Protection, Yangzhou University.

[0014] 1.2 Medium

[0015] Bacterial medium: YGPA medium, glucose 10g, peptone 5g, yeast extract 5g, pH 7.2. Fungal medium: PDA medium.

[0016] 1.3 Shake flask culture of strains

[0017] The strains grown on the slant were inserted i...

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Abstract

The invention relates to an application of BZ6-1 bacterial strain in preparing drugs for treating plant peanut bacterial wilt, comprising the following steps of: inoculating the plate activated BZ6-1 to 100mL of YGPA liquid culture mediums, carrying out overnight culture at 30 DEG C for 18 hours; inoculating to 250mL of YGPA culture solutions according to the volume ratio of 1 percent; carrying out shaking culture for 18 hours, and then carrying out spore staining every two hours; checking the formation of spores; and when the quantity of thalli reaches 1*109cfu<mL-1> and the formation rate of the spores reaches 80 percent, adsorbing into a matrix according to the proportion of 50 percent (V / M, unit: mL / g) to prepare a solid inoculant. The BZ6-1 bacterial strain has higher inhibiting effect on peanut ralstonia solanacearum.

Description

1. Technical field [0001] The invention belongs to the technical field of plant pesticides, and in particular relates to the application of a BZ6-1 strain in the preparation of medicines for treating plant peanut bacterial wilt. 2. Background technology [0002] Peanut bacterial wilt is a soil-borne bacterial disease caused by Ralstonia solanacearum [Hayward A C. Biology and Epidemiology of Bacterial wilt Caused by Pseudomonassolanacearum[J].Annu Rev Phytopathol , 1991, 29:65-87.], is one of the most harmful, widely distributed, and most costly plant diseases in the world, and is called the "cancer" of plants. At present, the prevention and treatment of this disease is mainly based on chemical means, but long-term and large-scale application of chemical pesticides is likely to cause drug resistance and environmental pollution [Yoshida S, Hiradate S, Tsukamoto T, et al. Antimicrobial activity of culture filterate of Bacillus amyloliquefaciens RC- 2isolated from mulberry leav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P1/00
Inventor 王小兵骆永明刘五星李振高
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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