Stem cell preparation for treating cirrhosis

A stem cell preparation and stem cell technology, applied in the field of stem cells and tissue engineering, can solve the problems of lack of donors and high cost, and achieve the effect of improving survival rate, improving the general state and liver function status, and promoting the recovery of liver pathology

Inactive Publication Date: 2011-04-27
UNION STEMCELL & GENE ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Orthotopic liver transplantation is an effective method for the treatment of end-stage liver cirrhosis, but there is a shortage of donors, high cost, and long-term administration of immunosuppressants after transplantation, so it is necessary to actively find effective treatments

Method used

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  • Stem cell preparation for treating cirrhosis
  • Stem cell preparation for treating cirrhosis
  • Stem cell preparation for treating cirrhosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Preparation of human umbilical cord placental mesenchymal stem cell preparation

[0020] After the qualified umbilical cord placenta is cut mechanically, it is digested into a single cell suspension by type II collagenase, washed to remove the collagenase residue, and the cell suspension is inoculated in a culture bottle of DMEM / F12 medium containing fetal bovine serum or cultured In the plate, when the fibroblasts reach 70-80% fusion, they are digested and passaged, and repeated passages in this way, so as to achieve the purpose of purifying and expanding the mesenchymal stem cells. Harvest the single-cell suspension for transplantation, and collect the 2nd-4th generation cells in strict accordance with the cryopreservation steps, cryopreserve and resuscitate for later use.

[0021] 1×10 human umbilical cord placental mesenchymal stem cells were collected 6 cells, 3×10 6 cells, 5×10 6 For each cell, prepare 1ml of mesenchymal stem cell preparation with...

Embodiment 2

[0024] Example 2: Modeling of liver cirrhosis rats

[0025] Diethylnitrosamine (DEN) induced model: Male SD rats, 3.5 weeks old, 90g, according to the volume ratio of DEN and sterile water 0.1‰, drink freely, until 4 weeks to establish a model.

[0026] Thioacetamide (TAA) induction model: male SD rats, 3.5 weeks, 90g, 0.3‰ of DEN and sterile water for 2 weeks, 0.45‰ for 3 weeks, 0.6‰ for 4 weeks, a total of 8 Zhou, the modeling was successful.

Embodiment 3

[0027] Example 3: Infusion experiment of mesenchymal stem cells derived from human umbilical cord placenta

[0028]1. Animals: Diethylnitrosamine (DEN)-induced model and thioacetamide (TAA)-induced model of male SD rats.

[0029] 2. Dosage: Each model rat was divided into 3 dosage groups, each given 1×10 6 cells / ml / time, 3×10 6 / ml / time, 5×10 6 / ml / time of cell dose, all in one infusion.

[0030] 3. Route: tail vein

[0031] 4. Timing of treatment:

[0032] DEN-induced model: After 4 weeks of successful modeling, stop drinking the drinking water prepared by DEN and start using the preparation prepared by MSC for 1 week.

[0033] TAA-induced model: After 8 weeks of successful modeling, stop drinking TAA-prepared drinking water and start using MSC preparations for 1 week.

[0034] 5. Effect evaluation:

[0035] (1) Mental state and behavioral ability: model group rats

[0036] (2) Liver function indexes: All rats in the transplantation group, model control group and norm...

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Abstract

The invention discloses a stem cell preparation for treating cirrhosis, which is prepared from stem cells from human umbilical cord placenta, normal saline containing seralbumin with concentration by volume being 0.5-5%, and low-molecular-weight heparin sodium with concentration being 50-701U/ml. When the stem cell preparation is transfused into a rat model with cirrhosis through a caudal vein, the effective dose of the stem cell preparation is 5*106 cells each time; and the effective dose of the stem cell preparation is 0.5-5*106 cells/ kg each time when the stem cell preparation is transfused into a patient with cirrhosis through a peripheral vein. After applied to rats with cirrhosis and patients with cirrhosis, the stem cell preparation can obviously improve the survival rate of the rats with cirrhosis and the patients with cirrhosis, retard and invert a cirrhosis process, improve a whole state and a liver function state, and promote pathological recovery of liver, thereby developing a new way to cell therapy for treating cirrhosis.

Description

technical field [0001] The invention belongs to the field of stem cells and tissue engineering, and relates to a new application of mesenchymal stem cells, in particular to a stem cell preparation for treating liver cirrhosis with mesenchymal stem cells derived from human umbilical cord placenta. technical background [0002] Liver cirrhosis is a chronic, progressive, diffuse change in the liver caused by various reasons, which develops from liver fibrosis caused by many reasons, including viral hepatitis, schistosome infection, chronic alcoholism, metabolic and genetic diseases, Liver congestion, cholestasis, circulatory disturbance, intestinal infection, malnutrition, drugs or chemical poisons, etc. In my country, viral hepatitis is the main disease, followed by schistosomiasis, but alcoholic liver cirrhosis tends to increase. Liver cirrhosis in the early stage can be reversed or no longer progressed after active prevention and treatment, but in the late stage, it will se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61K35/50A61P1/16A61K35/44A61K35/51
Inventor 洪敬欣韩俊领钱其军李琳芳胡还章刘剑
Owner UNION STEMCELL & GENE ENG
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