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Method for quickly slicing fish tissues

A slicing and rapid technology, applied in the preparation of test samples, etc., can solve the problem of blurred tissue and cell images, and achieve the effect of good cell staining effect, firm adhesion and easy operation.

Inactive Publication Date: 2011-05-18
SUZHOU UNIV
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  • Abstract
  • Description
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Problems solved by technology

However, the main disadvantage of the frozen section method is that since the biological tissue sample is not fixed, after the tissue block is trimmed and frozen sectioned, various enzymes and zymogens in cells and tissues (such as those in cell lysosomes) After the enzyme) is activated, the biological tissue is hydrolyzed to a certain extent; at the same time, after cutting into 5-8? Slices quickly melted
These shortcomings lead to very fast cryosection operations. At the same time, the morphology and fine structure of biological cells and tissues after staining are not clear and blurred.
[0005]Therefore, it is necessary to study a method for rapid sectioning of fish tissue, which can not only shorten the time of paraffin sectioning, but also solve the problems of frozen section tissue, cell lysis, microscope image blur problem

Method used

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  • Method for quickly slicing fish tissues
  • Method for quickly slicing fish tissues
  • Method for quickly slicing fish tissues

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Embodiment 1

[0031] A method for rapidly slicing fish tissue, the fish tissue comprising: fish intestinal tissue, grass carp hepatopancreas, and channel catfish skin, specifically comprising the following steps:

[0032] (1) Fixing method of tissue block: Quickly trim the tissue block to be sliced ​​to a size of 5-8 mm x 5-8 mm wide x 3 mm high, wash it in 0.75% normal saline, absorb the surface moisture with absorbent paper, and immediately put it into Bouin's The cells were fixed in solution, and sliced ​​after 4-6 hours. Bouin's solution: Add 25ml of formalin (40% formaldehyde) to 75ml of saturated picric acid, then add 5ml of glacial acetic acid and mix well.

[0033] (2) Frozen section method: further trimming of the tissue block: use a thin blade to further trim the fixed tissue block into a size of 2 mm × width 2 mm × height 1-2 mm; turn on the power of the cryostat 30 minutes before formal sectioning, and set the sample stage ( (cold stage) temperature is -25°C, blade temper...

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Abstract

The invention belongs to the field of microscopic tissue slice, particularly relates to a method for quickly slicing and dyeing fish tissues. The method comprises the following steps: 1) solidifying the fish tissues for 4-6 hours by using stationary liquid, cutting the solidified fish tissues into slice tissue blocks being 2mm long, 2mm wide and 1-2mm high, soaking the slice tissue blocks for 10-20 seconds in egg white which is diluted by using distilled water at the volume ratio of 1:1, freezing the slices, and pasting slices, and 2) placing for 3-5 minutes at room temperature after being pasted, dyeing, airing, and sealing by using neutral gum and cover glass for observing with microscope. Through the method, the time is shortened and the problems of freezing slice tissues, cell dissolution and blurry image under microscope are solved.

Description

technical field [0001] The invention belongs to the field of microscopic tissue sections, and in particular relates to a technical method for rapid sectioning and staining of fish tissues. Background technique [0002] Microscopic observation is an effective experimental technique for observing and analyzing the surface morphology, basic structure, and fine structure of biological tissues. It is also an important experimental method for rapid diagnosis of clinical pathology and some morphological research work. However, biological tissue needs to be sectioned and stained before effective microscopic observation and analysis can be performed. Conventional experimental sectioning techniques include paraffin sectioning and frozen sectioning. [0003] The general procedure of the paraffin section technique is that the biological tissue is pre-fixed, and the protein has been denatured, generally irreversibly denatured; after that, the biological tissue is soaked in paraffin (fill...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30
Inventor 叶元土蔡春芳殷永风张宝彤向朝林
Owner SUZHOU UNIV
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