Preparation method of trachea substitute

Abstract

The invention provides a preparation method of a trachea substitute. The preparation method comprises the following steps: eliminating antigens of an animal-derived tracheal tissue; compounding vascular endothelial growth factors (VEGF) with the outer wall of the trachea substitute; and compounding epidermal growth factors (EGF) with the inner wall of the substitute, wherein antigen elimination is carried out on a cartilage layer on the outer wall of a trachea and a lower muscular layer of a mucous layer on the inner wall of the trachea. The trachea substitute prepared by the method is derived from the animal tracheal tissue, has a specific C-shaped cartilaginous ring structure of a natural trachea, is formed by connecting compact connective tissues, has elasticity, can extend and contract longitudinally, and can moderately expand when gas passes through the substitute; and the prepared trachea substitute can keep the trachea unobstructed for a long time after transplantation, can notcollapse, and has good integration effect with a host without obvious rejection reaction. The preparation method has the advantages of simpleness, wide raw material source and low cost; and the medical cost can be reduced and the economic burden of patients can be lightened by means of large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering medical biomaterials, and in particular relates to a preparation method of a natural decellularized trachea substitute. Background technique [0002] Tracheal stenosis caused by diseases such as tracheal tumors and tracheal scars can cause severe breathing difficulties and even death by suffocation. Such tracheal lesions sometimes require resection of a segment of the trachea followed by tracheal reconstruction. When the length of the resected trachea exceeds 6 cm, a tracheal substitute or an allogeneic trachea is required to replace the original diseased trachea. Commonly used tracheal substitutes mainly include tracheal substitutes derived from autologous tissue, allogeneic trachea and artificial trachea. [0003] The sources of autologous tissue and allogeneic trachea are limited, and allogeneic trachea has the risk of immune rejection, while artificial trachea is difficult to have...

AI Technical Summary

Problems solved by technology

The author of the article also admits that the chondrocytes in the porcine tracheal tissue cannot be removed according to the method he introduced, and it can be observed that intact chondrocytes exist in the cartilage ring structure of the decellularized tracheal scaffold; subsequently, in the process of repairing the dog tracheal defect , the cartilage ring structure of the trachea has obvious absorption. With the extension of implantation time, the cartilage ring will be further absorbed and degraded, and the effect of tracheal reconstruction cannot be achieved.

It can be seen that the decellularization method of porcine tracheal tissue in the article failed to effectively remove the antigenic components in the cartilage ring, which may cause immune rejection of the recipient, resulting in degradation of the cartilage ring and failure of tracheal reconstruction

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