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Method for enriching and purifying common phellinus fungus general flavone in common phellinus fungus

A technology of total flavonoids and Phellinus, which is applied in the direction of fungal medical raw materials, diseases, drug combinations, etc., can solve the problems of low extraction purity and low extraction rate, and achieve high extraction purity, large adsorption capacity, and separation effect obvious effect

Active Publication Date: 2012-08-22
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a method for enriching and purifying the total flavonoids of Phellinus phellidis using the coupling method of batch distillation-macroporous adsorption resin

Method used

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Examples

Experimental program
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Embodiment 1

[0025] Embodiment 1: A method for enriching and purifying the total flavonoids of Phellinus Phellinus using the coupling method of batch distillation-macroporous adsorption resin, the steps are as follows:

[0026] (1) After crushing the dried Phellinus Phellinus, through a 60-mesh sieve, reflux extraction with ethanol with 5 times the volume of Phellinus Phellinus powder, 1.5 hours each time;

[0027] (2) suction-filtering the Phellinus Phellinus extract treated through step (1), and recovering ethanol from the filtrate under reduced pressure to obtain the primary extract;

[0028] (3) The pH value of the obtained primary extract was adjusted to 2.0 with acid, left to stand, suction filtered, and washed with water until neutral, and concentrated under reduced pressure to obtain the crude extract of total flavonoids of Phellinus japonica;

[0029] (4) Adsorb the obtained Phellinus Phellinus total flavonoids crude extract on a macroporous adsorption resin chromatography column,...

Embodiment example 2

[0034] Implementation Case 2: A method for enriching and purifying the total flavonoids of Phellinus Phellinus using batch distillation-macroporous adsorption resin coupling method, the steps are as follows:

[0035] (1) After crushing the dried Phellinus Phellinus, through a 30-mesh sieve, reflux extraction with ethanol with 8 times the volume of Phellinus Phellinus powder, 2 hours each time;

[0036] (2) suction-filtering the Phellinus Phellinus extract treated through step (1), and recovering ethanol from the filtrate under reduced pressure to obtain the primary extract;

[0037] (3) The pH value of the obtained primary extract was adjusted to 2.3 with acid, left to stand, suction filtered, and washed with water until neutral, and concentrated under reduced pressure to obtain the crude extract of total flavonoids of Phellinus japonica;

[0038] (4) The obtained Phellinus Phellinus total flavonoids crude extract is adsorbed with a macroporous adsorption resin, and the macrop...

Embodiment example 3

[0043] Implementation Case 3: A method for enriching and purifying the total flavonoids of Phellinus Phellinus using batch distillation-macroporous adsorption resin coupling method, the steps are as follows:

[0044] (1) After crushing the dried Phellinus Phellinus, pass it through an 80-mesh sieve, and extract 3 times with ethanol whose volume is 10 times that of Phellinus Phellinus powder, each time for 2.5 hours;

[0045] (2) suction-filtering the birch bark extract treated in step (1), and recovering ethanol from the filtrate under reduced pressure to obtain the initial extract;

[0046] (3) The pH value of the obtained primary extract was adjusted to 2.5 with acid, left to stand, filtered by suction, washed with water until neutral, concentrated under reduced pressure to obtain the crude extract of total flavonoids of Phellinus japonica;

[0047] (4) Adsorb the obtained Phellinus Phellinus total flavonoids crude extract on a macroporous adsorption resin chromatography col...

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Abstract

The invention belongs to the field of natural organic chemistry and relates to a method for enriching and purifying common phellinus fungus general flavone in common phellinus fungus by using a batch distillation-macroporous absorption resin coupling method. The method is characterized in that the common phellinus fungus general flavone is separated and purified by applying a macropore adsorptiontechnology, the adsorption selectivity to the common phellinus fungus general flavone is better, the separation effect is remarkable, the extraction purity is high, the semi-finished product with more than 35 percent of the common phellinus fungus general flavone and the final product with above 90 percent of the common phellinus fungus general flavone can be obtained, and the defects of relatively lower conventional extraction rate and low extraction purity are overcome. Macroporous absorption resin with stable physicochemical property, large surface area, higher switching speed, high mechanical strength, strong contamination resistance and good thermal stability is selected, and the common phellinus fungus general flavone is selectively absorbed from a solution through physical adsorption, thus the method has the advantages of high adsorption, high deabsorption speed and larger adsorption capacity.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for purifying total flavonoids of Phellinus phellidis, in particular to a method for enriching and purifying total flavonoids of Phellinus phellidis using a coupling method of intermittent distillation and macroporous adsorption resin. Background technique: [0002] Phellinus, derived from the fruiting body of the fungus Basidiomycetes Polyporaceae Polyporaceae Phellinus igniarius (L.ex Fr) Quel. Also known as: Phellinus igniarius (L.ex Fr) Quel Acupunculus. Born on the trunks of broad-leaved trees such as poplar, willow, birch, oak, rhododendron, and Sizhaohua, causing white rot in the heartwood, perennial; beneficial to the five internal organs, soft and hard, detoxification, hemostasis, blood circulation, and stomach and diarrhea. Cure mainly gonorrhea, uterine bleeding, leukorrhagia, accumulation of lumps in the abdomen, addiction to drinking, diarrhea due to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/06A61P15/00A61P37/04
Inventor 姚德坤姚德利闫峰黄运生姚航郑君
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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