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Co-feeding lipid nano-delivery system for medicine carrying

A drug delivery system, lipid nanotechnology, applied in the direction of powder delivery, antineoplastic drugs, drug combination, etc., to achieve the effect of controllable dosage and simple delivery method

Active Publication Date: 2011-07-06
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Using liposomes to simultaneously contain anti-cancer polypeptides that can restore p53 activity and chemotherapeutic drugs or gene drugs or radiotherapy drugs, etc., in order to achieve the purpose of synergistic anti-cancer, there are no similar reports at home and abroad.

Method used

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  • Co-feeding lipid nano-delivery system for medicine carrying
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  • Co-feeding lipid nano-delivery system for medicine carrying

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Example 1 Preparation of co-transport lipid nano drug delivery system carrying TRAIL gene and anticancer polypeptide Stoppin

[0040] The positively charged anti-cancer polypeptide Stoppin and TRAIL gene were mixed in pure water at a ratio of positive and negative charges of 1:2, and vortexed for 1 minute. The whole process was carried out at room temperature to obtain nano Level complex, particle size analyzer shows that its size is about 150nm. Weigh dioleic acid glycerol phosphatidylethanolamine (DOPE) and dioleoyl-3-trimethylaminopentane (DOTAP) in a molar ratio of 1:1, mix them and dissolve them in chloroform, and rotate under vacuum Evaporation yields a cationic lipid film. According to the ratio of polypeptide / plasmid / cationic lipid charge ratio of 1:2:4, the formed polypeptide / plasmid complex is added to the cationic lipid membrane and hydrated. After hydration is completed, the lipid drug delivery system with a size of about 200 nm is obtained after being rep...

Embodiment 2

[0042] Example 2 Preparation of co-delivery lipid nano drug delivery system carrying other gene drugs and anticancer polypeptide Stoppin

[0043] The preparation method of the co-delivery lipid nano drug delivery system carrying Survivin-targeted small interfering RNA and anti-cancer polypeptide Stoppin is the same as that in Example 1.

[0044] The preparation method of the co-delivery lipid nano drug delivery system carrying the antisense nucleic acid against Bcl-2 and the anticancer polypeptide Stoppin is the same as that in Example 1.

Embodiment 3

[0045] Example 3 Preparation of Co-transport Lipid Nano-Drug Delivery System Encapsulating Anti-cancer Polypeptide Stingin and Radiotherapy Drug Acridine Borate

[0046] 1. Liposome passive drug loading method to entrap anti-cancer polypeptide

[0047] Weigh hydrogenated soybean phosphatidyl choline (HSPC), cholesterol, polyethylene glycol-distearoylphosphatidylethanolamine (PEG-DSPE) and the coupling agent capable of targeting brain gel at a mass ratio of 55:45:1:1. PEG-DSPE (cylic-RGD-PEG-DSPE) of RGD cyclic peptide of glioblastoma cells was used as a membrane material to prepare liposomes. The above materials and the anticancer polypeptide Stingin were dissolved in chloroform and pure water respectively, wherein the mass ratio of the polypeptide to the phospholipid material was 1:7, and the volume ratio of the aqueous phase to the organic phase was 1:5. The aqueous and organic phases were mixed and sonicated until a stable emulsion formed. After the obtained emulsion was ...

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Abstract

The invention belongs to the field of medicinal preparation, relates to a co-feeding delivery system, and particularly relates to a co-feeding lipid nano-delivery system for entrapping anticancer polypeptide and gene or chemotherapeutics or radiopharmaceutical simultaneously. The delivery system consists of a target searching material, a lipid component and medicaments, ensures that different medicaments are entrapped to the lipid nano-delivery system together by sufficiently utilizing character difference among components or entrapping through static electricity or a method of passively combining and actively carrying, and can simultaneously deliver the anticancer polypeptide and genetic medicament capable of restoring activity of tumor suppressor protein p53 or chemotherapeutics or radiopharmaceutical into a target cell so as to achieve the aim of co-therapy. By virtue of in-vitro activity evaluation, the system is proved to be superior to that of delivering single component at the same time, the anticancer activity of the system is remarkably improved, and the system has definite co-therapy effect.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and relates to a co-transport drug delivery system, in particular to a co-transport lipid nano-drug delivery system that simultaneously entraps anti-cancer polypeptides and gene drugs, chemotherapy drugs or radiotherapy drugs. The drug delivery system can simultaneously deliver anticancer polypeptides and gene drugs or chemotherapeutic drugs or radiotherapy drugs into target cells to achieve the purpose of synergistic treatment. Background technique [0002] Based on the complexity of the pathogenesis of cancer, in the past few decades of research, the combination of drugs with different anti-cancer mechanisms to achieve "1+1>2" similar "cocktail" therapy has gradually been accepted, and has become the current basic research And an important direction of clinical treatment of cancer. The compatibility of drugs in combination is a key issue, and a reasonable preparation can truly ach...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K47/34A61K38/16A61K45/00A61K48/00A61P35/00A61K47/24A61K47/59
Inventor 陆伟跃李翀
Owner FUDAN UNIV
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