Culture medium for quickly separating and identifying banana fusarium oxysporum

Abstract

The invention discloses a culture medium for quickly separating and identifying banana fusarium oxysporum. The culture medium is a group of composites and is characterized in that: every 1,000 milliliters of water solution comprise the following components: 180-220g of culture medium potato, 0.4-0.6g of brochantite, 0.5-0.7g of epsom salt, 0.08-0.12g of monopotassium phosphate, 18-20g of agaragar, 10-15 milliliters of 95 percent alcoholic solution, 2.5-3.0g of 75 percent dexon crystalline powder and 0.5-0.8g of streptomycin sulphate. The culture medium has the advantages of reasonable raw material ingredients, vigorous growth of pathogenic bacteria of fusarium oxysporum, formation of a white bacterial colony, remarkable bacterial colony characteristic and high occurrence rate of the bacterial colony; a flat plate can be manufactured inversely without performing high-pressure steam sterilization on the culture medium, a culture dish does not need high-pressure steam sterilization after being cleaned and dried, and sterile working is not required during inoculation; and the raw materials are readily available, the effect is enhanced, and the cost is saved.

Description

Technical field [0001] The invention relates to the field of microbes, in particular to a rapid isolation and identification medium for Fusarium wilt of banana. It can be used for pathogen identification of banana tissue cultured seedlings at seedling stage, and can also be used for isolating pathogenic bacteria of banana Fusarium wilt in soil, which has practical significance in agricultural production. Background technique [0002] At present, Fusarium wilt of banana is a worldwide soil-borne vascular disease caused by the infection of Fusarium oxysporumf.sp.cubense (FOC). serious loss. Banana Fusarium wilt can be divided into 4 physiological races according to different identified hosts, among which race 4 is pathogenic to almost all banana cultivars, which seriously threatens the sustainable and healthy development of the banana industry. So far, there is no ideal medicament that can effectively prevent and treat the disease. Therefore, it is urgent to strengthen the i...

AI Technical Summary

Problems solved by technology

Although domestic scholars Han Baokun, Du Yanhua, etc. disclosed the culture medium for isolating Fusarium oxysporum under non-sterile operation in Journal of Phytopathology, 2001, 31 (4), it is extremely difficult to count Fusarium oxysporum from soil. It is easy to be contaminated by miscellaneous bacteria, and the culture medium of Fusarium wilt of banana cannot be separated from the soil in non-sterile operation, and the purpose of detecting pathogenic bacteria in large quantities and quickly cannot be achieved; foreign scholars use glucose, peptone, and yeast extract as the growth medium of Fusarium spp. In addition to basic nutrients, many trace elements are added. The production cost of these culture media is generally high, and they need to be sterilized at high temperature, and all operations must be carried out under sterile conditions, which brings inconvenience to a large number of rapid detection of pathogenic bacteria, and the effect is often not ideal

Images