Culture medium for quickly separating and identifying banana fusarium oxysporum

A technology of banana fusarium wilt and culture medium, applied in the field of microorganisms, can solve the problems of not reaching a large number of pathogenic bacteria, rapid detection of pathogenic bacteria, easy contamination of Fusarium oxysporum by miscellaneous bacteria, inconvenience of rapid detection of pathogenic bacteria, etc. Typical, colony-characterized effects

Inactive Publication Date: 2011-07-06
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although domestic scholars Han Baokun, Du Yanhua, etc. disclosed the culture medium for isolating Fusarium oxysporum under non-sterile operation in Journal of Phytopathology, 2001, 31 (4), it is extremely difficult to count Fusarium oxysporum from soil. It is easy to be contaminated by miscellaneous bacteria, and the culture medium of Fusarium wilt of banana cannot be separated from the soil in non-sterile operation, and the purpose of detecting pathogenic bacteria in large quantiti...

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  • Culture medium for quickly separating and identifying banana fusarium oxysporum
  • Culture medium for quickly separating and identifying banana fusarium oxysporum

Examples

Experimental program
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preparation Embodiment 1

[0019] Preparation Example 1 The ratio of raw materials for the culture medium in this example consists of:

[0020] Potato 180g; Copper sulfate pentahydrate 0.4g; Magnesium sulfate heptahydrate 0.5g;

[0021] Potassium dihydrogen phosphate 0.08g; agar 18g; 95% alcohol solution 10ml;

[0022] 2.5g of 75% dexon crystalline powder; 0.5g of streptomycin sulfate; 1000ml of aqueous solution.

[0023] The preparation method of present embodiment culture medium is:

[0024] 1. Take 180g of peeled potatoes and cut them into small pieces, put them in a pot, add 700ml of aqueous solution into the pot and cook for 20 minutes, then take the filtrate for later use;

[0025] 2. Add 0.4g of copper sulfate pentahydrate, 0.5g of magnesium sulfate heptahydrate, 0.08g of potassium dihydrogen phosphate, and 18g of agar to the spare filtrate prepared in step 1, and boil to dissolve. When it cools to 45°C, add 10ml of 95% alcohol solution respectively , 2.5g of 75% dexon crystalline powder, 0.5g...

preparation Embodiment 2

[0026] Preparation Example 2 The ratio of raw materials for the culture medium components in this example is as follows:

[0027] Potato 200g; copper sulfate pentahydrate 0.5g; magnesium sulfate heptahydrate 0.6g;

[0028] Potassium dihydrogen phosphate 0.1g; agar 19g; 95% alcohol solution 10ml;

[0029] 75% dexon crystalline powder 2.8g; streptomycin sulfate 0.75g; aqueous solution 1000ml.

[0030] The preparation method of present embodiment culture medium is:

[0031] 1. Take 200g of peeled potatoes and cut them into small pieces, put them in a pot, add 750ml of aqueous solution into the pot and cook for 20 minutes, then take the filtrate for later use;

[0032] 2. Add 0.5 g of copper sulfate pentahydrate, 0.6 g of magnesium sulfate heptahydrate, 0.1 g of potassium dihydrogen phosphate, and 19 g of agar to the spare filtrate prepared in step 1, and boil to dissolve. When it cools to 45°C, add 10 ml of 95% alcohol solution respectively , 2.8g of 75% dextran crystalline po...

preparation Embodiment 3

[0033] Preparation Example 3 The ratio of the raw materials of the culture medium components in this embodiment consists of:

[0034] 220g potatoes; 0.6g copper sulfate pentahydrate; 0.7g magnesium sulfate heptahydrate;

[0035] Potassium dihydrogen phosphate 0.12g; agar 20g; 95% alcohol solution 15ml;

[0036] 75% dexon crystalline powder 3.0g; streptomycin sulfate 0.8g; aqueous solution 1000ml.

[0037] The preparation method of present embodiment culture medium is:

[0038] 1. Take 220g of peeled potatoes and cut them into small pieces, put them in a pot, add 800ml of aqueous solution into the pot and cook for 20 minutes, then take the filtrate for later use;

[0039] 2. Take the filtrate and add 0.6g of copper sulfate pentahydrate, 0.7g of magnesium sulfate heptahydrate, 0.12g of potassium dihydrogen phosphate, and 20g of agar to dissolve, and add 15ml of 95% alcohol solution and 75% of Dixon crystal when it is cooled to 50°C. Powder 3.0g, streptomycin sulfate 0.8g, the...

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Abstract

The invention discloses a culture medium for quickly separating and identifying banana fusarium oxysporum. The culture medium is a group of composites and is characterized in that: every 1,000 milliliters of water solution comprise the following components: 180-220g of culture medium potato, 0.4-0.6g of brochantite, 0.5-0.7g of epsom salt, 0.08-0.12g of monopotassium phosphate, 18-20g of agaragar, 10-15 milliliters of 95 percent alcoholic solution, 2.5-3.0g of 75 percent dexon crystalline powder and 0.5-0.8g of streptomycin sulphate. The culture medium has the advantages of reasonable raw material ingredients, vigorous growth of pathogenic bacteria of fusarium oxysporum, formation of a white bacterial colony, remarkable bacterial colony characteristic and high occurrence rate of the bacterial colony; a flat plate can be manufactured inversely without performing high-pressure steam sterilization on the culture medium, a culture dish does not need high-pressure steam sterilization after being cleaned and dried, and sterile working is not required during inoculation; and the raw materials are readily available, the effect is enhanced, and the cost is saved.

Description

Technical field [0001] The invention relates to the field of microbes, in particular to a rapid isolation and identification medium for Fusarium wilt of banana. It can be used for pathogen identification of banana tissue cultured seedlings at seedling stage, and can also be used for isolating pathogenic bacteria of banana Fusarium wilt in soil, which has practical significance in agricultural production. Background technique [0002] At present, Fusarium wilt of banana is a worldwide soil-borne vascular disease caused by the infection of Fusarium oxysporumf.sp.cubense (FOC). serious loss. Banana Fusarium wilt can be divided into 4 physiological races according to different identified hosts, among which race 4 is pathogenic to almost all banana cultivars, which seriously threatens the sustainable and healthy development of the banana industry. So far, there is no ideal medicament that can effectively prevent and treat the disease. Therefore, it is urgent to strengthen the i...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12Q1/04C12R1/77
Inventor 黄俊生景晓辉吴伦英吴琳区小玲薛玉潇朱利林杨腊英
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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