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Method, reagent and kit for separating nucleic acids

A nucleic acid and reagent technology, applied in the field of nucleic acid separation, which can solve the problems of easy contamination and long time consumption

Active Publication Date: 2014-07-16
IND TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, no matter what, all nucleic acid isolation methods need to go through 5 steps of dissolution (lysis), extraction, precipitation, cleaning, and eluting, which take a long time and are easy to contaminate

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  • Method, reagent and kit for separating nucleic acids
  • Method, reagent and kit for separating nucleic acids
  • Method, reagent and kit for separating nucleic acids

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Embodiment Construction

[0031] The present invention provides a method for isolating and / or purifying nucleic acid. In the method of the present invention, after mixing and reacting a separation reagent with a biological sample, the nucleic acid in the biological sample can be isolated.

[0032] In one embodiment, the separation reagent of the present invention includes 1-40 wt% polyethylene glycol (PEG), preferably 5-20 wt% polyethylene glycol.

[0033] In another embodiment, the separation reagent of the present invention comprises more than 30 wt% low molecular weight alcohols, preferably 40-60 wt% low molecular weight alcohols, wherein the molecular weight of the low molecular weight alcohols is about 30-100 g / mol. In one embodiment, the low molecular weight alcohol is ethanol.

[0034] In another embodiment, the separation reagent of the present invention may include polyethylene glycol and low molecular weight alcohols at the same time.

[0035] The separation reagent of the present invention...

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Abstract

The invention provides a simple and quick method for separating nucleic acids, which comprises the following steps: providing a nucleic acid separation reagent, mixing the nucleic acid separation reagent with a biological sample containing one or multiple nucleic acids, and separating the nucleic acids from the biological sample by one step under a proper reaction condition without pretreating the sample. The reagent contains 1-40 wt% of polyethylene glycol (PEG), and / or higher than 30 wt% of low-molecular-weight alcohol, salt and surfactant. The nucleic acids can be combined with a solid-phase vector by changing the solubility of the nucleic acids, thereby separating the nucleic acids. In addition, the invention also provides a reagent and kit for separating nucleic acids.

Description

technical field [0001] The present invention relates to a method for isolating nucleic acid, and particularly relates to a single-step nucleic acid isolation method and nucleic acid isolation reagent. Background technique [0002] In recent years, with the rise of molecular diagnosis, pharmacogenomics, translational medicine, and criminal identification, the demand for various nucleic acid analyzes has also increased rapidly. However, no matter what kind of nucleic acid analysis is to be performed, it must first require high-purity nucleic acid and convenient , Rapid nucleic acid isolation method. [0003] During the analysis, since the substance to be tested exists in the liquid sample for a very short time, and the impurities in the sample will affect the accuracy of the detection, a nucleic acid separation technology has been developed, which uses a solid phase carrier to bind nucleic acid. , or use the inner wall of the column as a solid phase carrier, then add microbea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10C07H21/00C07H1/06
Inventor 江佩馨巫昆展苏钰婷林嘉芸苏秀卿林玉娟
Owner IND TECH RES INST