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Automatic enzyme-linked immune analysis analyzer

An enzyme-linked immunoassay, fully automatic technology, applied in the field of enzyme-linked immunoassay methods and automatic enzyme-linked immunoassay analyzers, can solve the problems of cumbersome reagent filling operations, affecting operators, and affecting test results

Active Publication Date: 2013-12-04
SHENZHEN YHLO BIOTECH
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  • Application Information

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Problems solved by technology

[0011] 2. Reagents for qualitative detection include sample diluent, washing solution, enzyme conjugate, substrate for enzyme reaction, chromogenic reagent, stop solution, positive control serum, and negative control serum. Quantitative reagents include diluent, washing There are 11 kinds of reagents, enzyme conjugates, enzyme reaction substrates, chromogenic reagents, stop solutions, at least 5 levels of standards or calibrators. Each detection reagent must be contained in a reagent bottle, and each used When using reagents, it is necessary to replace the suction nozzle to add samples, reagents, and washing liquid to the reaction container—the microwell of the microplate. Not only are there many types and quantities of reagent bottles, but also the operation of adding reagents is extremely cumbersome. Even if the automatic enzyme immunoassay analyzer is used or the loading volume of each reagent is changed, the number of reagents used cannot be reduced
[0013] 4. The detection reagents and samples are open in the detection process, which may easily cause cross-contamination between various reagents or samples and affect the detection results or directly affect the operator
[0014] 5. A detection reagent can only detect one item. If two or three items are to be detected, three different detection reagents or kits are required, and the detection operations are performed separately
[0015] 6. The optical system of the instrument only includes one set of multiple wavelength filters and one set of photoelectric sensing devices, and lacks two or more sets of optical filters including multiple wavelengths and their corresponding photoelectric sensing devices.
[0024] 15. It is not possible to perform single-item or multi-item testing according to the sample situation in real time

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  • Automatic enzyme-linked immune analysis analyzer
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Embodiment Construction

[0090] The present invention will be explained in more detail below in conjunction with the accompanying drawings.

[0091] Please combine Figure 1 to Figure 12 , an enzyme-linked immunoassay method of the present invention, the method includes a reagent device and a fully automatic enzyme-linked immunoassay analyzer. The detected sample is placed on the reagent device and inserted into the slot of the circular reaction plate of the automatic enzyme-linked immunosorbent analyzer, and the operating system software and data management system software of the automatic enzyme-linked immunosorbent analyzer are run, and the sample to be tested can be processed. Qualitative or quantitative detection of items such as antigens or antibodies in the test; when in use, as long as there is one sample, the corresponding items can be tested without wasting reagents.

[0092] The above-mentioned circular reaction disk is a disk, with the center of the disk as the radiation point, and severa...

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Abstract

The invention relates to an enzyme-linked immune analysis method and a fully-automatic enzyme-linked immune analyzer. The analyzer comprises a rack component, a washing component, a sample adding / reagent adding component, a heating and temperature control component, a fluid path system, an input and output device, an optical measurement component and a control system, wherein the rack component comprises a rack soleplate; a pillar is arranged on the rack soleplate; a lower fixed plate and an upper fixed plate are arranged on the pillar from bottom to top in sequence; a circular reaction plate is arranged between the lower fixed plate and the upper fixed plate; the circular reaction plate is connected with the upper fixed plate and the lower fixed plate through a central shaft; a first motor is fixedly arranged at the lower part of the lower fixed plate; and the first motor is used for driving the circular reaction plate to rotate through a first transmission mechanism. When in use, the enzyme-linked immune analysis method and the fully-automatic enzyme-linked immune analyzer can carry out the detection of corresponding projects without wasting reagent through only one sample; and detection reagents need not to be respectively contained in different reagent bottles, so that the operation is very convenient, and the operation error is not easily caused, thereby ensuring the accuracy of the detection results.

Description

technical field [0001] The invention relates to an enzyme-linked immunoassay method and a fully automatic enzyme-linked immunoassay analyzer. The method and instrument are mainly used for the antigenic substances in biological samples such as human body sample serum, sputum, urine, feces, bile, etc., such as antigens of various pathogenic microorganisms, or antibodies produced by these pathogenic microorganism antigens infecting the body, and autoimmune diseases such as systemic lupus erythematosus, phospholipid syndrome, rheumatoid arthritis, scleroderma, autoimmune liver disease, Sjogren's syndrome, systemic vasculitis, polymyositis and dermatomyositis, autoimmune Autoantibodies in the blood of patients with thyroid disease, gastrointestinal disease and other diseases can be quantitatively or qualitatively detected by enzyme-linked immunosorbent method. Background technique [0002] As one of many immunoassay techniques, enzyme-linked immunoassay has been widely used in c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N35/00G01N35/02G01N21/01
Inventor 何林刘清波阳辉伍坚
Owner SHENZHEN YHLO BIOTECH
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