Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of euphorbia peplus plant extract for preparing antitumor medicament

A plant extract and anti-tumor drug technology, applied in the field of traditional Chinese medicine extraction, can solve the problems of high price, limited application and promotion, etc., and achieve the effect of reducing production cost and shortening the cycle

Inactive Publication Date: 2013-04-10
南京春秋生物工程有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Giovanni Appendino has reported the preparation technology (J.Nat.Prod.1999.62,76-79) of ingenol equal to 1999, because it uses expensive hexanaphthene and highly toxic chemicals potassium cyanide, so severely limited Its application promotion

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of euphorbia peplus plant extract for preparing antitumor medicament
  • Preparation method of euphorbia peplus plant extract for preparing antitumor medicament
  • Preparation method of euphorbia peplus plant extract for preparing antitumor medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] a) Select 100 kg of 100 kg of daughter fruit and crush it into granules.

[0047] b) Reflux extraction: the crushed plant particles were extracted with methanol for 3 times under reflux. For the first time, 600L of methanol was added for reflux extraction for 2 hours, for the second time, 500L was added for reflux extraction for 1.5 hours, and for the third time, 500L was added for reflux extraction for 1.5 hours.

[0048] c) Concentration under reduced pressure: the above-mentioned three extracts are combined and pumped into a concentration tank for concentration under reduced pressure to a thin paste, and released for cooling.

[0049] d) Extraction: add 60L petroleum ether to extract the above thin paste. The petroleum ether in the upper layer was separated from the water and insoluble solid in the lower layer.

[0050]e) Back extraction: collect the above-mentioned petroleum ether liquid, place it in an extraction tank, add 20L of acetonitrile, stir for 5 minutes ...

Embodiment 2

[0058] a) Select 100kg of Gansui and grind it into granules.

[0059] b) Reflux extraction: the crushed plant particles were extracted with methanol for 3 times under reflux. Add 500L reflux extraction for 2 hours for the first time, add 400L reflux extraction for 1.5 hours for the second time, add 400L reflux extraction for 1.5 hours for the third time,

[0060] c) Concentration under reduced pressure: the above-mentioned three extracts are combined and pumped into a concentration tank for concentration under reduced pressure to a thin paste, and released for cooling.

[0061] d) Extraction: add 50L of petroleum ether to extract the above thin paste. The petroleum ether in the upper layer was separated from the water and insoluble solid in the lower layer.

[0062] e) Back extraction: collect the above-mentioned petroleum ether solution, put it in an extraction tank, add 16L of acetonitrile, stir for 5 minutes and let it stand for stratification, release the lower layer of ...

Embodiment 3

[0070] a) Select 100 kg of 100 kg of daughter fruit and crush it into granules.

[0071] b) Reflux extraction: the crushed plant particles were extracted three times with 95% ethanol under reflux. Add 600L reflux extraction for 2 hours for the first time, add 500L reflux extraction for 1.5 hours for the second time, add 500L reflux extraction for 1.5 hours for the third time,

[0072] c) Concentration under reduced pressure: the above-mentioned three extracts are combined and pumped into a concentration tank for concentration under reduced pressure to a thin paste, and released for cooling.

[0073] d) Extraction: add 60L petroleum ether to extract the above thin paste. The petroleum ether in the upper layer was separated from the water and insoluble solid in the lower layer.

[0074] e) Back extraction: collect the above-mentioned petroleum ether liquid, put it in an extraction tank, add 20L of acetonitrile, stir for 5 minutes and let it stand for stratification, release th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting a euphorbia peplus plant extract for preparing an antitumor medicament from a euphorbia peplus plant. The method comprises the following process steps of: smashing raw materials; performing reflux extraction; concentrating under reduced pressure; extracting; performing back-extraction; performing alkaline hydrolysis; adjusting PH value to 7; concentrating under reduced pressure; performing silica gel column chromatography; performing gradient elution with petroleum ether and ethyl acetate; concentrating; crystallizing; and drying. The method has the advantages of capability of realizing industrial production scale because industrially readily-available separating materials and reagents are adopted, high product quality, content of over 99.9percent and strong international market competitiveness.

Description

technical field [0001] The invention relates to a method for extracting traditional Chinese medicines, in particular to a method for preparing an extract of Euphorbia genus Euphorbiaceae for preparing antitumor drugs, in particular to a method for preparing high-purity euphorbiol. Background technique [0002] Tumor (neoplasm) is the body under the action of various tumorigenic factors, the cells of local tissues lose their normal regulation of their growth at the gene level, resulting in clonal abnormal proliferation and the formation of new organisms. Since the 1970s, the incidence and mortality of malignant tumors in my country have been on the rise. But in recent years, the morbidity and mortality of some major tumors abroad have begun to show a downward trend, which is mainly due to the continuous research and progress of medicine. [0003] Australia's Peplin Biotech Ltd and Queensland Institute of Medical Research jointly isolated and screened the natural compound ing...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07C49/737C07C45/81
Inventor 贾晓东董云发
Owner 南京春秋生物工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com