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Preparation method of sulfated corn bran dietary fiber

A technology of dietary fiber and sulfuric acid esterification is applied in the fields of renewable resource utilization and food processing, which can solve the problems of high toxicity of pyridine and difficult to obtain, high corrosiveness of concentrated sulfuric acid, etc. High, mild reaction conditions

Pending Publication Date: 2021-11-09
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the use of concentrated sulfuric acid to prepare sulfated polysaccharides is more likely to cause polysaccharide degradation, and concentrated sulfuric acid is more corrosive
The chlorosulfonic acid-pyridine method is easy to operate, and the sulfated corn bran polysaccharide prepared by the o-phenanthroline-Fe2+ oxidation method found that the scavenging effect of the sulfated corn bran polysaccharide on hydroxyl radicals was significantly improved compared with that before esterification ( Li Fuyi et al. Chinese Journal of Cereals and Oils, 2014, 29(11): 104-107, 112), but compared with the sulfur trioxide-pyridine method, the latter has a milder reaction and higher reaction efficiency, but pyridine is more toxic and difficult to obtain
And the latest research report sulfamic acid-N, N-dimethylformamide (DMF) is a kind of very high esterification reaction system of reaction efficiency, is used for preparing sulfated polysaccharide operation easier, and for corn bran fiber There are few reports on the sulfation of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Weigh 20g of corn bran, add 200mL of n-hexane, heat in an ultrasonic water bath at 50°C for 2h, and then dry. Add high-temperature-resistant α-amylase, and perform destarch treatment in a water bath at 90-95°C for 1 hour. Filter the filter residue and add alkaline hydrogen peroxide solution to adjust the pH to 11.50, and stir for 1 h. After cooling, centrifuge at 4000r / min for 15min. Take the supernatant, adjust the pH to 4.0-4.5 with concentrated hydrochloric acid, centrifuge at 4000r / min for 15 minutes, add excess ethanol to the supernatant for alcohol precipitation, let stand for 12 hours to remove protein with alkaline protease, dialyze, and freeze-dry to obtain corn bran dietary fiber , the water holding capacity is 72%, and the swelling force is 4.6mL / g.

[0027] Weigh 0.5g of corn bran dietary fiber, dissolve it in 60mL of N,N-dimethylformamide (DMF), add 1.0g of sulfamic acid, bathe in water at 90°C for 3h, cool in an ice bath after the reaction is completed, ...

Embodiment 2

[0029] Weigh 20g of corn bran, add 200mL of n-hexane, heat in an ultrasonic water bath at 50°C for 2h, and then dry. Add high-temperature-resistant α-amylase, and perform destarch treatment in a water bath at 90-95°C for 1 hour. Use iodine solution to check whether the starch is completely removed. If it does not turn blue, filter the filter residue and add alkaline hydrogen peroxide solution, adjust the pH to 11.50, and heat with magnetic stirring for 1 hour. After cooling, centrifuge at 4000r / min for 15min. Take the supernatant to adjust the pH value to 4.0-4.5, centrifuge at 4000r / min for 15min, take the supernatant and add excess ethanol for alcohol precipitation, let it stand for 12h to remove protein with alkaline protease, dialyze, and freeze-dry to obtain corn bran dietary fiber, The water holding capacity is 72%, and the swelling power is 4.6mL / g.

[0030] Weigh 0.5g corn husk soluble polysaccharide, dissolve it in 50mL N,N-dimethylformamide (DMF), add 2.0g sulfamic...

Embodiment 3

[0032] Weigh 20g of corn bran, add 200mL of n-hexane, heat in an ultrasonic water bath at 50°C for 2h, and then dry. Add high-temperature-resistant α-amylase, and destarch in a water bath at 90-95°C for 1 hour. Filter the filter residue and add alkaline hydrogen peroxide solution to adjust the pH to 11.50, and heat with magnetic stirring for 1 h. Centrifuge at 4000r / min for 15min. Take the supernatant to adjust the pH to 4.0-4.5, centrifuge at 4000r / min for 15min, add excess ethanol to the supernatant for alcohol precipitation, let stand for 12h to remove protein with alkaline protease, dialyze, and freeze-dry to obtain corn bran dietary fiber. The hydraulic power is 72%, and the swelling power is 4.6mL / g.

[0033] Weigh 0.5g corn bran dietary fiber, dissolve it in 60mL N,N-dimethylformamide (DMF), add 2.0g sulfamic acid, bathe in 90℃ water for 2.5h, cool in ice bath, adjust pH with 2mol / L NaOH 7.0, running water dialysis for 48 hours, deionized water dialysis for 24 hours,...

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Abstract

The invention provides a preparation method of sulfated corn bran dietary fibers, relates to the field of comprehensive utilization of grain and oil byproducts, and can be applied to the fields of utilization of renewable resources of foods and the like. The preparation method mainly comprises the following steps: degreasing corn bran by adopting an alkali extraction method, removing starch, performing alcohol extraction, dialyzing and the like to obtain the corn bran dietary fiber; and performing sulfation on the corn bran dietary fibers by adopting a sulfamic acid-N, N-dimethylformamide (DMF) method, including the steps of esterification, dialysis with running water, alcohol precipitation, freeze drying and the like, so as to obtain the sulfated corn bran dietary fibers with higher substitution degree and high antioxidant activity. The preparation method is high in raw material utilization rate, mild in process and suitable for large-scale production, and has great development and application prospects.

Description

technical field [0001] The invention relates to a preparation method of sulfated corn bran dietary fiber, belongs to the field of comprehensive utilization of grain and oil by-products, and is applied in the fields of food processing, renewable resource utilization and the like. Background technique [0002] Maize is one of the most widely distributed crops in the world, and its yield is second only to rice and wheat. Corn bran is a by-product of corn deep processing, accounting for 15% of corn grains. Its main components are hemicellulose, cellulose and lignin, etc. It has the effects of lowering blood fat, improving immunity, and anti-tumor. It is a good edible and medicinal product. Phytoactive polysaccharides. At present, a small part is woven into handicrafts, and most of them are used for feed production or discarded, or as biofuels, causing environmental pollution and a large waste of food resources. Therefore, the reuse of waste corn husks can not only reduce the e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/21C08B37/00
CPCA23L33/21C08B37/00A23V2002/00A23V2200/30A23V2250/5116
Inventor 李静周中凯范超中
Owner TIANJIN UNIV OF SCI & TECH
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