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Tissue culture and propagation method and induction medium of tinospora capillipes gagnep

A technology for inducing culture medium and golden fruit group, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve problems such as no successful reports, achieve low natural reproduction rate, solve the problem that wild resources are decreasing day by day, The effect of promoting the rooting of single seedlings

Inactive Publication Date: 2012-10-10
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tissue culture rapid propagation is a modern biotechnology, which has been successfully applied to crops such as banana, Luo Han Guo, sweet tea, and dendrobium, and has been applied to production, but there has been no successful report on the tissue culture propagation of Lavender.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Cluster bud induction

[0027] Take the young stems of the golden fruit, first wash off the surface dirt with detergent, then rinse with running water for 15 minutes, then treat with 75% ethanol on the ultra-clean workbench for 30 seconds, rinse with sterile water once, and then rinse with 0.1% HgCl 2 Disinfect for 10 minutes, rinse with sterile water for 5 times; blot the explants dry with sterile filter paper, and plant in MS with 0.46mg / L of 6-BA, 0.24mg / L of NAA, GA 3 0.8mg / L, KT 0.15mg / L, pH adjusted to 4.5 on the culture medium, at a temperature of 26±3°C, with a light time of 12-14h.d -1 , light intensity 20~30μmol.m -2 .s -1 Under the condition of culturing for 22 days, 25.2 times of clustered buds were obtained on average, and the clustered buds were thicker, green, and grew slightly slower.

[0028] (2) Rooting induction

[0029] When the clustered shoots grow to 3-4cm, divide the clustered shoots into small clusters or single seedlings and inoculate t...

Embodiment 2

[0033] (1) Cluster bud induction

[0034] Take the young stems of the golden fruit, first wash off the surface dirt with detergent, then rinse with running water for 15 minutes, then treat with 75% ethanol on the ultra-clean workbench for 30 seconds, rinse with sterile water once, and then rinse with 0.1% HgCl 2 Disinfect for 8 minutes, rinse with sterile water for 5 times; blot the explants dry with sterile filter paper, and plant in MS with 0.50 mg / L 6-BA, 0.20 mg / L NAA, GA 3 1.0mg / L, KT 0.20mg / L, pH adjusted to 4.7 on the culture medium, at a temperature of 26±3°C, and a light time of 12~14h.d -1 , light intensity 20~30μmol.m -2 .s -1 Under the condition of culturing for 20 days, an average of 27 times of cluster buds can be obtained, the cluster buds are strong, the leaves are thick green, plump, and the growth is good.

[0035] (2) Rooting induction

[0036] When the clustered shoots grow to 3-4cm, divide the clustered shoots into small clusters or single seedlings a...

Embodiment 3

[0040] (1) Cluster bud induction

[0041] Take the young stems of the golden fruit, first wash off the surface dirt with detergent, then rinse with running water for 15 minutes, then treat with 75% ethanol on the ultra-clean workbench for 30 seconds, rinse with sterile water once, and then rinse with 0.1% HgCl 2 Disinfect for 9 minutes, rinse with sterile water for 5 times; blot the explants dry with sterile filter paper, and plant in MS with 0.55mg / L of 6-BA, 0.18mg / L of NAA, GA 3 1.3mg / L, KT 0.23mg / L, pH adjusted to 5.0 on the culture medium, at a temperature of 26±3°C, and a light time of 12~14h.d -1 , light intensity 20~30μmol.m -2 .s -1Under the condition of culturing for 18 days, an average of 25.8 times the cluster buds were obtained, the cluster buds were thicker, the leaves were dark green, and the growth was faster.

[0042] (2) Rooting induction

[0043] When the clustered shoots grow to 3-4cm, divide the clustered shoots into small clusters or single seedlings...

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PUM

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Abstract

The invention discloses a tissue culture and propagation method and induction medium of tinospora capillipes gagnep. In the invention, MS (Murashige and Skoog), 6-BA (6-Benzylaminopurine), NAA (Naphthylacetic Acid), GA3 (Gibberellin)and KT (kinetin) are taken as the induction medium of the tinospora capillipes gagnep. Cluster buds and action enzymes in body cells of the tinospora capillipes gagnep can be activated by the combination of the four growth hormones under the environment with suitable consistency and subacidity, the germination and the growth of tinospora capillipes gagnep explantsare prompted, and the thick cluster buds can be formed after the explants are cultured for 20 days, thus the propagation is increased to 25 times. In the invention, the MS, IBA (Indolebutyric Acid) and the NAA are further taken as a rooting induction medium, thus a single seedling of the tinospora capillipes gagnep effectively prompts to take root and form a new plant. By the tissue culture and propagation method of the tinospora capillipes gagnep by utilizing the two induction mediums, the tinospora capillipes gagnep can be quickly and effectively propagated, the problems that the wild resources of the tinospora capillipes gagnep are reduced day by day and the natural propagation rate is low are solved, and powerful provenance guarantee is provided for deep development of the medicinal value of the tinospora capillipes gagnep.

Description

technical field [0001] The invention relates to the field of plant tissue culture and propagation, in particular to a method for tissue culture and propagation of Lavender oleracea and its induction medium. Background technique [0002] Tinospora capillipes Gagnep. is an evergreen twining vine of the genus Tinospora genus Tinospora, mainly produced in Sichuan, Hunan, Guangxi, Hubei, Guizhou and other provinces. It is one of the root plants of the traditional Chinese medicine Lavender. It has the effects of clearing away heat and detoxifying, relieving sore throat and relieving pain. The medicinal tuber root of Jinguolan is a kind of medicinal material recorded in the "Chinese Pharmacopoeia" over the years. Phinerine, Dauricine, and Magnoline. It is widely used in the folk to treat stomach pain. Modern pharmacological studies have shown that jinguolan has obvious anti-inflammatory, analgesic, anti-bacterial, anti-tumor, hypoglycemic, anti-stress, anti-ulcer and detoxificati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 冯世鑫马小军潘丽梅王晓峰闫志刚黄曦
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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