Method for extracting tea saponin by microbial fermentation

A technology of microbial fermentation and tea saponin, applied in fermentation and other directions, can solve the problems of long production cycle, high operating cost, low purity, etc., and achieve the effects of reducing viscosity, improving exudation rate and high purity

Inactive Publication Date: 2013-03-27
安徽大别山科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although ultrafiltration and column chromatography have high purity, they require specific fillers and ultrafiltration membrane cleaning, and the operating costs are high, making it difficult to implement industrially.
The water leaching method has simple process and low cost, but high energy consumption, long production cycle, dark product color and low purity
There are problems such as environmental pollution, product quality and safety in the organic solvent extraction method
Therefore, there is no good method for extracting tea saponin with high yield and high purity from tea dregs in the world.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] 1. Choose Ganoderma lucidum as the fermentation strain

[0019] 15keV keV for Ganoderma lucidum, 2.6×10 15 ~3.9×10 15 ions / cm 2 Injected N + injection. Moreover, the ganoderma lucidum after ion implantation was cultured, and its mycelium growth and laccase metabolism were investigated. A mutant strain that can grow on the tea meal substrate and produce laccase has been selected and bred.

[0020] Perform 2.6×10 on Ganoderma lucidum 15 ~3.9×10 15 ions / cm 2 Dose range N + Injection, a high proportion of positive mutant strains can be obtained. Direct inoculation with common Ganoderma lucidum is also acceptable.

[0021] 2. Ganoderma lucidum is separated and cultured on a plate, and the components of the plate separation medium are: glucose 2.0g, peptone 0.2g, MgSO 4 ·7H 2 O 0.1g, KH 2 PO 4 0.2g, 1L of water, after shaking culture at 28°C and 150r / min for 6 days, 24.18g / L mycelium biomass can be obtained, which can meet the needs of fermentation inoculum and o...

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Abstract

The invention discloses a method for extracting tea saponin by microbial fermentation, which comprises the following steps of: taking tee seed cakes as a main culture matrix and bran and the like as auxiliary materials, vaccinating microorganisms for solid state fermentation so as to realize efficient seepage of the tea saponin, and extracting a product from the seeped tea saponin by an alcohol leaching method. The method organically combines a microorganism conversion technology and a tea saponin extraction technology; the obtained tea saponin has high yield and purity; and the method has simple process, low cost and no pollution to environments.

Description

technical field [0001] The invention relates to the field of deep processing of agricultural by-products, in particular to a method for extracting tea saponin by microbial fermentation. Background technique [0002] Tea saponin, also known as tea saponin, is a class of saponin compounds extracted from Camellia plants (such as tea, camellia, camellia oleifera). Tea saponin is an important daily chemical raw material with excellent surface activity. It can be used to make high-grade pollution-free detergents, cleaning agents, foaming agents, emulsifiers, insecticides, shampoos, etc. At the same time, tea saponin also has a variety of biological activities, such as anti-osmosis, anti-inflammatory, analgesic, and insecticidal effects. Its surface activity and biological activity research has made great progress, its many functions and efficacy have been scientifically verified, and its industrial application prospect is very broad. [0003] In 1979, my country first separated ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/00
Inventor 王永法
Owner 安徽大别山科技开发有限公司
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