Traceability detection method of trans-Bar gene paddy rice

Abstract

The invention belongs to trans-Bar gene paddy rice in agricultural products and the technical field of the safety and the traceability detection of a primary processed product of the trans-Bar gene paddy rice. The detection method comprises following steps of: (1) extracting paddy rice genome DNA (deoxyribonucleic acid); (2) performing the multi-PCR (polymerase chain reaction) amplification to 35S promoter, NOS (nitric oxide synthase) terminator, SPS (sucrose phosphate synthase) paddy rice endogenous gene and Bar gene by taking the paddy rice genome DNA extracted by the step (1) as a templatewith primers from SEQ ID No: 1 to SEQ ID No: 8; and (3) performing the gel electrophoresis to the amplified product, and analyzing and judging a result. The method has the characteristic of being high in flux, and the qualitative detection of the trans-Bar gene paddy rice can be completed for once when the detection accuracy is guaranteed, so that the method is simple in operation, the false positive detection result is reduced, the detection time is shortened, the detection cost is reduced, and the method has a practical significance.

Description

technical field [0001] The invention belongs to the technical field of safety and traceability detection of transgenic Bar gene rice and its primary processed products in agricultural products, and specifically relates to a traceability detection method of transgenic Bar gene rice. Background technique [0002] With the rapid development of my country's economy and the improvement of people's awareness of health, environmental protection and food safety, food traceability and safety testing technology, especially genetically modified food testing technology, has become an urgent problem to be solved. my country is the largest rice producer in the world, with an annual rice planting area of ​​over 30 million hectares, accounting for 20% of the world's total rice planting area, and a total rice output of nearly 200 million tons, accounting for 35.7% of the world's total rice production. As the world's largest rice consumer, rice production has an extremely important impact on t...

AI Technical Summary

Problems solved by technology

"Detection is the premise of supervision, so it is necessary to establish a set of effective detection measures before the commercialization of Bar genetically modified rice to cooperate with future supervision work, and PCR detection technology is currently the mainstream transgenic detection technology in the world, but ordinary The detection efficiency of single-plex PCR is low, and it is difficult to meet the needs of high-throughput in actual detection. Although nested PCR has high efficiency, it has high requirements for experimental conditions and operators. Therefore, a simple, accurate and efficient transformation method was developed. Bar gene rice multiplex PCR detection method appears to be very necessary

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