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Application of new morchella costata M8-13 liquid fermentation substance in development of health-care products and medicaments

A technology for morel mushrooms and health products, which is applied in the fields of application, fermentation, and antibacterial drugs, and can solve problems such as the lack of morel polysaccharide system research

Inactive Publication Date: 2013-07-17
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no systematic research on morel polysaccharides in China

Method used

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  • Application of new morchella costata M8-13 liquid fermentation substance in development of health-care products and medicaments
  • Application of new morchella costata M8-13 liquid fermentation substance in development of health-care products and medicaments
  • Application of new morchella costata M8-13 liquid fermentation substance in development of health-care products and medicaments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The collection of embodiment 1 hickory chick

[0028] Clean the surface of Morchella collected from the Qinghai-Tibet Plateau, wipe it with 95% ethanol, put it in a clean plate or jar, and put it in an oven at 40°C. After a large number of spores pop out, pick the spores , Streak on a PDA (20% potato, 2% sucrose, 1.5-2% agar, natural pH) plate. After culturing at 25°C for one day, observe with a microscope. After a single spore germinates, the culture medium at the germination site is cut off and transferred to another PDA plate. Stored in PDA slants.

[0029] The inventor carries out liquid culture to these bacterial strains, and all bacterial strains are activated on PDA solid medium earlier, and liquid fermentation medium (KH 2 PO 4 0.1%, MgSO 4 0.1%, sucrose 2%, peptone 0.5%), and then it was transferred to 150mL Erlenmeyer flask in the liquid fermentation medium of 60mL, and cultivated under the condition of 100r / min at 25°C.

[0030] In the cultured 24 morel ...

Embodiment 2

[0031] Example 2 Screening strains with high biomass and exopolysaccharide production:

[0032] The strains sealed in liquid paraffin on the slant were transferred to PDA solid medium, and cultured under constant temperature and light at 25°C, among which the M8 series and M51009 were cultured for 5 days. After the strains were activated in the PDA medium, they were transferred to a 150mL Erlenmeyer bottle liquid 60mL liquid fermentation medium, and each strain was used as 3 parallel controls, and cultured at 25°C and 100r / min.

[0033] (1) Precipitation of exopolysaccharide:

[0034] Aspirate 4mL of the morel fermentation broth cultured to the 3rd day, 4th day, and 5th day, centrifuge at 3500r / min for 15min, absorb 3mL supernatant in each centrifuge tube, add 9mL absolute ethanol, and store at -10°C Settled for 24 hours.

[0035] (2) Determination of exopolysaccharides:

[0036] Centrifuge the precipitated polysaccharide at 3500r / min for 15min, discard the supernatant, add...

Embodiment 3

[0044] Example 3 Determination of intracellular polysaccharides:

[0045] Weigh about 0.020g of dried Morchella mycelium, add a total of 1mL of water twice to grind, boil for extraction for 30min, and then centrifuge at 2500r / min for 20min after cooling. Take the supernatant and put it in a centrifuge tube, add 1mL water to the residue, boil and extract for 30min, collect the supernatant and put it in the same centrifuge tube as above. Add 7mL of absolute ethanol to the supernatant to precipitate the polysaccharide, centrifuge at 2500r / min for 20min after precipitation for 30min, discard the supernatant, add 3.5mL of ethanol to the residue, stir and wash, then centrifuge and discard the supernatant. Dissolve the precipitate with hot water at about 80°C, and after cooling, make the volume to 2mL. Determine polysaccharides by anthrone sulfuric acid method, as above, see Figure 5 .

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Abstract

The invention relates to newly separated morchella M8-13 and application thereof to development of health-care products and medicaments.

Description

technical field [0001] The invention relates to a newly isolated hickory chick M8-13 and its application in the development of medicine and health products. Background technique [0002] Morel (Morchella sp.) is a world-renowned (medicine) edible fungus, known as "land fish" in the United States, and some developed countries in Europe and the United States believe that it is a high-level tonic for human nutrition. It is distributed all over the world. Morchella fruiting bodies contain a variety of chemical components. According to analysis and determination, every 100g of dry Morchella contains 12.869g of water, 3.829g of fat, and 22.069g of protein. The protein content is 2 times that of fungus and 1.5 times that of shiitake mushrooms (long Zheng Hai et al., 1997), and it is easily absorbed by the human body, which is unmatched by other high-protein foods. Morchella contains 10 kinds of amino acids, especially the amino acids necessary for the human body, which are 25% to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/04A23L1/30A61K36/06A61P3/06A61P31/04A61P35/00A61P37/02C12R1/645
Inventor 谢占玲戚永跃
Owner QINGHAI UNIVERSITY
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