Method for improving yield of L-threonine produced by fermentation

A technology of threonine yield and threonine, which is applied in the field of improving the yield of L-threonine by fermentation, can solve the problems of high equipment investment and production and operation cost, and achieves low production cost, improved yield and easy operation. Effect
CN102181502AInactive Publication Date: 2011-09-14齐齐哈尔龙江阜丰生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
齐齐哈尔龙江阜丰生物科技有限公司
Publication Date
2011-09-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for improving yield of L-threonine produced by fermentation, comprising the following steps: obtaining the L-threonine by adopting escherichia coli engineering bacteria through shaking culture, seeding tank amplification culture and fermentation tank culture, wherein, in the fermentation culture process: pure oxygen is utilized to replace partial compressed air to provide sterile air for threonine fermentation; the pH value is controlled to be 7.0-7.2 through adding liquid ammonia; and the residual sugar concentration is controlled to be 0.1-0.5% through adding glucose liquid the mass concentration of which is 600g / L-800g / L. In the method provided by the invention, under the condition of not adding any extra equipment, partial compressed air is replaced through adding the pure oxygen periodically, thus improving the dissolved oxygen level in the fermentation process, and greatly improving the yield and the transformation ratio of the L-threonine. The whole process has simple operational process and lower production cost, and is suitable for industrial production.
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Description

technical field

[0001] The invention belongs to the technical field of amino acid production by industrial fermentation, and in particular relates to a method for improving the yield of L-threonine produced by fermentation. Background technique

[0002] Among the eight essential amino acids required by humans and animals, threonine is the fourth amino acid after methionine, lysine, and tryptophan, and is widely used in food, animal husbandry, and pharmaceutical industries. The production methods of threonine mainly include chemical synthesis, protein hydrolysis and microbial fermentation. With the development of genetic engineering technology, the increase of industrial microbial biological information, especially the establishment of industrial biological carrier systems, from the late 1970s, researchers in the former Soviet Union began to construct threonine strains with genetic engineering technology, which is an excellent The screening of the L-threonine producing strai...

Claims

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