Method for determining tobacco leaf tissue free water content

A measurement method and water content technology, applied in the direction of weighing by removing certain components, can solve the problems of large measurement data, poor repeatability, and large errors, and achieve the effect of simple equipment, high accuracy, and good repeatability

Inactive Publication Date: 2011-11-02
TOBACCO RES INST CHIN AGRI SCI ACAD
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Problems solved by technology

[0003] The purpose of the present invention is to overcome the deficiencies of the existing Abbe refractometer method that requires many measurement data, large errors, and poor repeatability, and provide a technical

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  • Method for determining tobacco leaf tissue free water content

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Embodiment example

[0013] Implementation case: Randomly take the fresh tobacco leaves in the middle of 8 varieties of the Qingdao Jimo Tobacco Leaf Farm Test Field of the Tobacco Research Institute of the Chinese Academy of Agricultural Sciences, and adopt the method of the present invention to measure the free water content:

[0014] In the field, 10 leaves of NC89, K326, Honghua Dajinyuan, Cuibi No. 1, Yunyan 85, China Tobacco 100, Dabaijin 599 and Coker 371, each with the same growth, were selected. A total of 300 small discs / variety were symmetrically punched by the holer on both sides of the blade (avoiding the main vein), weighed with an electronic balance with an accuracy of 0.1 mg, and then randomly placed into 15 numbered weighing bottles ( 20 tablets per bottle), then add 6 times the mass concentration of the leaves to soak in 60% sucrose solution, place in a dark place, cover tightly, shake gently from time to time, after soaking for 6 hours, take out the leaves, and use wet gauze and ...

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Abstract

The invention discloses a method for determining tobacco leaf tissue free water content. According to the invention, a mass variation of leaf tissues before sucrose solution immersion and after sucrose solution immersion are directly determined by an electronic balance, and a content of free water in leaves is calculated according to the mass difference. According to the invention, a mass variation of leaf tissues before and after sucrose solution immersion is directly determined with a weighting method, and a content of free water in leaves is then calculated. In prior arts, with a traditional Abbe refractometer method, the determining process is relatively complicated, the quantity of determination data is relatively large, the repeatability is poor, and errors are relatively severe. With the method provided by the present invention, the defects are solved, and a difficulty in quick and accurate determination of tobacco leaf tissue free water content is overcome.

Description

technical field [0001] The invention belongs to a method for measuring free water content in crop leaf tissue, in particular to a method for measuring free water content in tobacco leaf tissue by using a weighing method. Background technique [0002] The content of free water and bound water in tobacco leaf tissue is not only an important index reflecting the growth status of tobacco, but also an important factor affecting the curing characteristics of flue-cured tobacco. When measuring its free water and bound water content, all adopt the Abbe refractometer method at present, and the inventor of the present application finds that this method not only requires measurement data many, and error is big, and repeatability is poor, needs a better method of measurement. On the basis of the traditional Abbe refractometer test principle, the inventors of the present application improved the measurement method and established a new measurement method, that is, the weighing method. ...

Claims

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Application Information

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IPC IPC(8): G01N5/04
Inventor 张忠锋王传义徐秀红许立峰王爱华倪超
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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