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Clove extract for killing nematode and preparation method thereof
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A technology for killing nematodes and extracts, which is applied in the field of clove killing nematode extract and its preparation, and can solve the problems such as no clove anhydrous ethanol extract.
Inactive Publication Date: 2013-05-01
BEIJING UNIV OF AGRI
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Problems solved by technology
There are few relevant reports on agricultural production, only a small amount of reports are used to kill acarids, and there are no related reports on the use of clove anhydrous ethanol extracts for the prevention and treatment of plant root-knot nematodes
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Embodiment 1
[0026] Embodiment 1, the acquisition of clove absolute ethanol extract
[0027] High-speed pulverizer, type 6202, manufactured by Beijing Huanya Tianyuan Machinery Technology Co., Ltd.; desktop three-frequency digital control ultrasonic cleaner, type KQ-500VDV, Kunshan Ultrasonic Instrument Co., Ltd.; rotary evaporator, type RE-2000, Shanghai Yarong Biochemical Co., Ltd. Instrument factory; stereo microscope, Motic SMZ-140, Motic Industrial Group Co., Ltd.; micro-adjustable pipette, GILSON, France; 96-well disposable cell culture plate, JET BIOFIL type, Canada; electric constant temperature incubator, SKP-02.420 type, Huangshi Hengfeng Medical Instrument Co., Ltd.; absolute ethanol (analytical grade), Beijing Chemical Plant;
[0028] The clove Eugenia caryophyllata used for testing was purchased from Beijing Tongrentang Pharmacy, belongs to Myrtaceae, and uses flower buds.
[0029] For the test root-knot nematode (Meloidogyne spp.), the diseased root of tomato root-knot nema...
[0045] The contact method is used for determination, and the specific steps are: take a 96-well cell culture plate, add 150 μL of the plant extract solution obtained by the method 1 of step 2 of Example 1 with a concentration of 20 mg / mL into each well, and then add an equal volume of Mix the root-knot nematode second-instar larvae suspension obtained in step 1, let it stand at 25°C, observe under a stereomicroscope after 12h, 24h and 48h respectively, and check whether the nematodes are dead or alive (needle contact method), stiff or curled. The worms were regarded as dead, and the corrected death rate of nematodes was calculated according to the formula. With sterile water as the control, each treatment was repeated 3 times, and the results were average ± standard error.
[0046]
[0047]
[0048]Data processing and analysis: The experimental data was analyzed and sorted by DPS2000, and the results were analyzed b...
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Abstract
The invention discloses a clove extract for killing nematode and a preparation method thereof. The invention provides a method for preparing a clove absolute ethyl alcohol extract, and the method comprises the following steps: 1) evenly mixing clove with absolute ethyl alcohol to obtain a mixture; 2) carrying out ultrasonic treatment on the mixture obtained in step 1) and then carrying out pumping filtration and condensation to obtain the clove absolute ethyl alcohol extract. According to experiments in the invention, absolute ethyl alcohol is used to extract clove, tests of toxic effects of the extract on Meloidogyne spp. are carried out, and the test results show that the extract has strong toxic activity on Meloidogyne spp.; therefore, it is indicated that the extract provided in the invention provides a development and application basis for preparations for controlling Meloidogyne spp. in the future. In the invention, active components capable of killing nematode are extracted from the plant clove which is natural and harmless, which allows reduced usage of chemical pesticides, thereby reducing pollution to agricultural products and environment.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a clove nematode extract and a preparation method thereof. Background technique [0002] Root-knot nematodes (Meloidogyne spp.) are obligate parasites on plant roots and are an important pathogen affecting agricultural production. [0003] Since the 1980s, the research on the use of plant extracts to control nematodes has gradually attracted people's attention. Up to now, there are about 102 families, 226 genera, and 316 species of nematode-killing plants, among which Asteraceae, Meliaceae, Bean family, Brassicaceae, Solanaceae, Poaceae, Euphorbiaceae, Poppyaceae, Apocynaceae, Mimosaceae, Liliaceae, etc., but the plant resources in nature are extremely rich, and many plants with nematicidal effects have not been discovered , the book "Poisonous Plants in China" has listed more than 1300 kinds of poisonous plants, many of which have insecticidal activity. Many plants can be obtained...
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