A kind of tissue culture method of strawberry seedling

A tissue culture and strawberry technology, applied in the field of tissue culture of strawberry seedlings, can solve the problems of lack of strawberry production technology, quality and yield decline, virus accumulation, etc., to improve the survival rate of culture, improve yield and quality, and increase economy. effect of benefit

Inactive Publication Date: 2011-11-30
DONGHAI COUNTY HUANGCHUAN CHUNFENG STRAWBERRY PRODION COOP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason is that on the one hand, there is a lack of high-quality strawberry seedling production technology, and the traditional method of raising seedlings is used in production, which leads to serious accumulation of viruses, degeneration of the original species of st

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1. A method for tissue culture of strawberry seedlings, the steps are as follows:

[0022] (1) Disinfection: Select the stolons produced by the female strawberry seedlings in spring as explants, wash them with water, then soak them in 70% alcohol for 11 seconds, wash them with sterile water, and then wash them with 0.1% Hgcl 2 After disinfection for 13-18 minutes, wash with sterile water;

[0023] (2) Primary culture: under a microscope, cut out 0.3mm sterilized stem tips of female strawberry seedlings and insert them into the culture medium for primary culture for 55 days; the temperature of the primary culture is 23°C, and they should be placed in the dark before culturing After cultivating for 5 days, cultivate according to 12 hours / day of light, disinfect the environment with 75% alcohol every 2 days during cultivation, and disinfect the environment once a week with ultraviolet rays;

[0024] (3) Proliferation and rooting culture: The strawberry seedlings ...

Embodiment 2

[0036] Example 2. A method for tissue culture of strawberry seedlings, the steps are as follows:

[0037] (1) Disinfection: Select the stolons produced by the female strawberry seedlings in spring as explants, wash them with water, then soak them in 70% alcohol for 13 seconds, wash them with sterile water, and then wash them with 0.1% Hgcl 2 After sterilizing for 18 minutes, wash with sterile water;

[0038] (2) Primary culture: Under a microscope, cut out 0.3mm sterilized shoot tips of female strawberry seedlings and insert them into the culture medium for primary culture for 60 days; the temperature of the primary culture is 25°C, and they should be placed in the dark before culturing After cultivating for 8 days, cultivate according to 12-14 hours / day of light, and disinfect the environment with 75% alcohol every 2 days during cultivation, and disinfect the environment once a week with ultraviolet rays;

[0039] (3) Proliferation and rooting culture: The strawberry seedli...

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PUM

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Abstract

The invention discloses a tissue culture method for a strawberry seedling. The method is characterized by comprising the following steps of: flushing a creeping stem grown on a spring female strawberry seedling, disinfecting, cleaning and performing primary culture at the temperature between 23-25 DEG C in dark environment for 5-8 days, and culturing according to lighting of 12-14 hours per day; and performing multiplication, rooting culture and domestication culture, wherein the relative air humidity is kept at 90 percent, the temperature is 15-20 DEG C and the shading rate is 75 percent during domestication culture. The tissue culture method for the strawberry seedling has high operability and is more reasonable; and according to the method the industrialization, professionalization andscale of the tissue culture of the strawberry seedling can be realized, the culture survival rate of the strawberry seedling can be increased, the yield and the quality of strawberry can be increased, and the economic efficiency of strawberry planting can be increased.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture method for strawberry seedlings. Background technique [0002] In recent years, there have been trends in strawberry production such as reduced yield, deformed fruit, late listing, and declining economic benefits. The main reason is that on the one hand, there is a lack of high-quality strawberry seedling production technology, and the traditional method of raising seedlings is used in production, which leads to serious accumulation of viruses, degeneration of the original species of strawberry, and a significant drop in quality and output; on the other hand, the cultivation method of yellow strawberry It is relatively backward and lacks efficient cultivation techniques that promote cultivation and early listing, thereby reducing the planting benefits of strawberry growers. Contents of the invention [0003] The technical problem to be solved by the present in...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 谢春光许长江赵士力仇从宇李廷月孙克仕
Owner DONGHAI COUNTY HUANGCHUAN CHUNFENG STRAWBERRY PRODION COOP
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