Complex enzyme bacterium preparation used for tobacco thread production and preparation method thereof

A technology of compound enzyme bacteria and preparations, applied in the direction of biochemical equipment and methods, tobacco, applications, etc., can solve the problem of high prices

Active Publication Date: 2013-07-17
农博士(福建)生物技术有限公司
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  • Abstract
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Problems solved by technology

But these additives are expensive, and most of them are chemical synthesis products

Method used

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  • Complex enzyme bacterium preparation used for tobacco thread production and preparation method thereof

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Embodiment Construction

[0021] In order to describe in detail the technical content, structural features, achieved objectives and effects of the present invention, the following will be described in detail in conjunction with the embodiments.

[0022] Method for preparing liquid A: liquid A is prepared by solid-liquid separation of the fermented liquid fermented by the bacteria stored in CGMCC registration number CGMCC No. 4121, as follows:

[0023] Strain activation: take 1 ring from the slant preservation tube and store in the bacterial lawn of the strain (hereinafter referred to as strain A) registered with CGMCC No. -250rpm shaker cultured for 24 hours, and prepared the cultured shake flask A;

[0024] Fermentation tank culture: Inoculate the well-cultured shake flask A into the fermentation tank to expand the cultivation. The inoculation method adopts flame inoculation, the inoculation amount is 1%, the cultivation temperature is 37°C, the stirring speed: 180-200rpm, the ventilation rate: 1:1, t...

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Abstract

The invention discloses a complex enzyme bacterium preparation used for tobacco thread production. The complex enzyme bacterium preparation contains a liquid A and a liquid B, wherein the liquid A is preserved in CGMCC (China general microbiological culture collection center), is enrolled as a fermentation liquor clear solution after fermentation of a strain and is numbered as CGMCC No.4121, and the liquid B is preserved in CGMCC, is enrolled as a fermentation liquor clear solution after fermentation of a strain and is numbered as CGMCC No.4120. By applying the preparation provided by the invention, indexes of cigarette such as colour and lustre, fragrance quality, fragrance quantity, strength, impurity gas, irritation, aftertaste, flammability, grey and the like are obviously improved.

Description

technical field [0001] The invention relates to a compound enzyme bacteria preparation for shredded tobacco production and a preparation method thereof. Background technique [0002] At present, additives are added in the cigarette production process to cover up the green and astringent miscellaneous gas generated when the shredded tobacco is lit, and to change the smoke produced when lit to the smoker. But these additives are expensive, and most of them are chemical synthesis products. Contents of the invention [0003] The technical problem mainly solved by the present invention is to provide an ecological, non-toxic and cheap compound enzyme bacteria preparation added in the production process of shredded tobacco of cigarettes, and a preparation method thereof. [0004] The compound enzyme bacteria preparation for shredded tobacco production provided by the present invention, the compound enzyme bacteria preparation contains liquid A and liquid B; [0005] Said liquid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24B15/20A24B15/28C12N1/00
Inventor 董少英杜秉海姚良同丁延芹林金新
Owner 农博士(福建)生物技术有限公司
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