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Protein for regulating growth of fruits and seeds and use thereof

A fruit and seed technology, applied in the fields of biotechnology and botany, can solve the problems of long breeding time, few, low efficiency, etc.

Inactive Publication Date: 2013-06-05
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the main breeding methods of tomato are still limited to the traditional method, the breeding time is long and the efficiency is low, and there are not many reports on tomato variety improvement using genetic engineering technology

Method used

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  • Protein for regulating growth of fruits and seeds and use thereof
  • Protein for regulating growth of fruits and seeds and use thereof
  • Protein for regulating growth of fruits and seeds and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0077] As an embodiment of the present invention, the gene encoding the ENS protein is cloned into an appropriate vector by conventional methods, and the recombinant vector with the foreign gene is introduced into the plant cells that can express the ENS protein , making the plant cell express ENS protein. Plants (over)expressing ENS proteins can be obtained by regenerating said plant cells into plants.

[0078] Preferably, a method for preparing transgenic plants is provided, comprising:

[0079] (1) transferring the gene encoding the exogenous ENS protein into plant cells, tissues, organs or tissues, and obtaining plant cells, tissues, organs or seeds transformed into the gene encoding the ENS protein; and

[0080] (2) Regenerating the plant cells, tissues, organs or seeds obtained in step (1) into which the gene encoding the exogenous ENS protein is regenerated into plant plants.

[0081] As a preferred example, the method comprises the steps of:

[0082] (s1) providing ...

Embodiment 1

[0095] Embodiment 1, plant material and growth condition

[0096] Arabidopsis wild-type Columbia-0 seeds were purchased from the American ABRC Center. Tomato VF36 seeds are provided by Institute of Plant Sciences, Shanghai Academy of Biological Sciences, Chinese Academy of Sciences (or refer to "Plant Tissue Culture Manual", the article "Transformation of tomato with Agrobacterium tumefaciens" (SHEILA McCORMICK Transformation of tomato with Agrobacterium tumefaciens.Plant Tissue Culture Manual, 1991, 1991, published in 1991, B6: 1-9)). Arabidopsis plants were grown in an artificial greenhouse at 21°C with 16 hours of light and 8 hours of darkness. The tomato plants were grown in an artificial greenhouse (day temperature 22-26° C., night temperature 15-20° C.) with 12 hours of light and 12 hours of darkness.

Embodiment 2

[0097] Embodiment 2, the cloning of Arabidopsis ENS gene

[0098] (1) Isolation of RNA

[0099] Take Arabidopsis leaf tissue, grind it with liquid nitrogen, put it into a 1.5ml centrifuge tube, add 1ml Trizol, shake it well, add 1 / 5 volume of chloroform, mix it upside down, let it stand at room temperature for 5 minutes, centrifuge at 12000rpm for 5 minutes, and take it out. Clear, add an equal volume of isopropanol and mix well, place at room temperature for 5 minutes, centrifuge at 12000rpm for 5 minutes, discard the supernatant, add pre-cooled 75% ethanol, centrifuge at 7500rpm for 5min, discard the supernatant, air dry for 5-10min, dissolve in DEPC water. The quality of total RNA was detected by agarose gel electrophoresis, and then the RNA content was determined on a spectrophotometer.

[0100] (2) RT-PCR

[0101] Using Arabidopsis total RNA as a template, TOYOBO reverse transcription reagent (ReverTra Ace) was used to synthesize the first strand of cDNA, and the rever...

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Abstract

The invention relates to a protein for regulating the growth of fruits and seeds and use thereof. The invention is the first to disclose the use of an ensembl gene (ENS gene) in regulation of the size of fruits or seeds of solanaceae plants. The invention also discloses the method for regulating the properties of fruits or seeds of solanaceae plants by using the ENS gene. When the method is used,the size of the fruits and seeds of tomatoes can be controlled, and new crop varieties of which the fruits and seeds are changed can be cultured quickly.

Description

technical field [0001] The invention belongs to the fields of biotechnology and botany; more specifically, the invention relates to proteins regulating fruit and seed development and applications thereof. Background technique [0002] Solanaceae (Solanaceae) is a more evolved group of flowering plants in the dicotyledonous class Chrysanthemum subclass, herb, shrub or small tree; the leaves are alternate or there are two-leaved twins of different sizes on the flowering branches, entire or individual Split or compound leaves without stipules; flowers bisexual or rare, radially symmetrical, solitary or arranged in cymes or bouquets; calyx 5-lobed or truncated, often persistent; corolla sympetal, various shapes, Lobes 5, often folded; stamens 5, rarely 4, inserted on coronal tube; ovary 2-loculed, or incomplete 1-4-loculed, 2-carpels not located on the median line and deviated, axial placenta There are very many ovules, rarely 1; ovules are anatropous, curved or transverse; fru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415A01H5/00A01H5/10
Inventor 郭房庆关媛沈琦王玉婷
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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