Isolation method of porcine cumulus stem cells

Abstract

The invention discloses a method for separating pig cumulus stem cells, which comprises the following steps of: extracting a cumulus-oocyte complex from a young sow, performing in-vitro maturation culture on oocyte cells, digesting and separating cumulus cells, collecting digested cumulus cells for culture, performing digestive passage inoculation onto a feeder layer when a cell colony appears and a larger cell colony is formed, and culturing in a cell factor-containing culture solution, wherein the separately cultured cumulus stem cells have bird nest cell growthform, round shapes and largernuclei, express unique markers Oct4, Nanog and SSEA1, and are subjected to in-vitro differentiation to form nerve cells, muscle cells and liver cells derived from three germ layers; and results show that the cumulus stem cells are separated from pig cumulus cells.

Description

[technical field] [0001] The invention relates to a method for isolating animal stem cells, in particular to a method for isolating porcine cumulus stem cells. [Background technique] [0002] Stem cells (Stem Cells) are cells that have not yet fully differentiated and matured, and have the potential to form cells of various tissues and organs under induced environmental conditions. They are called "universal cells" by the medical community. Stem cells have a wide range of uses and involve many fields of medicine. The extensive clinical application of stem cells and their derived tissues and organs will produce a brand-new medical technology, which is to regenerate normal or even young tissues and organs of the human body, so that human beings can use their own or other people's stem cells or derived from stem cells new tissues and organs to replace their diseased or aging tissues and organs. Stem cell research will provide a broader application prospect in clinical medicin...

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