Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Isolation method of porcine cumulus stem cells

A technology of cumulus cells and separation methods, applied in the direction of non-embryonic pluripotent stem cells, animal cells, vertebrate cells, etc., can solve the problem of isolating stem cells, etc.

Inactive Publication Date: 2011-12-07
ANHUI AGRICULTURAL UNIVERSITY
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there has been no report on the isolation of stem cells from the ovaries of animals.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Isolation method of porcine cumulus stem cells
  • Isolation method of porcine cumulus stem cells
  • Isolation method of porcine cumulus stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] 1 Test materials and methods

[0017] 1.1 Test material

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for separating pig cumulus stem cells, which comprises the following steps of: extracting a cumulus-oocyte complex from a young sow, performing in-vitro maturation culture on oocyte cells, digesting and separating cumulus cells, collecting digested cumulus cells for culture, performing digestive passage inoculation onto a feeder layer when a cell colony appears and a larger cell colony is formed, and culturing in a cell factor-containing culture solution, wherein the separately cultured cumulus stem cells have bird nest cell growthform, round shapes and largernuclei, express unique markers Oct4, Nanog and SSEA1, and are subjected to in-vitro differentiation to form nerve cells, muscle cells and liver cells derived from three germ layers; and results show that the cumulus stem cells are separated from pig cumulus cells.

Description

[technical field] [0001] The invention relates to a method for isolating animal stem cells, in particular to a method for isolating porcine cumulus stem cells. [Background technique] [0002] Stem cells (Stem Cells) are cells that have not yet fully differentiated and matured, and have the potential to form cells of various tissues and organs under induced environmental conditions. They are called "universal cells" by the medical community. Stem cells have a wide range of uses and involve many fields of medicine. The extensive clinical application of stem cells and their derived tissues and organs will produce a brand-new medical technology, which is to regenerate normal or even young tissues and organs of the human body, so that human beings can use their own or other people's stem cells or derived from stem cells new tissues and organs to replace their diseased or aging tissues and organs. Stem cell research will provide a broader application prospect in clinical medicin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/074
Inventor 曹鸿国章孝荣蒲勇杨盼张运海刘亚陶勇方富贵李运生任春环张子军丁建平刘洪瑜
Owner ANHUI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com