A preparation of Bacillus pumilus for shredded tobacco

A technology for microbial preparations and bacteria, applied in the field of microbial preparations, can solve the problems that have not yet been discovered, and achieve the effects of reducing irritation, facilitating large-scale industrial production, and having good development and application prospects.

Inactive Publication Date: 2011-12-21
HONGYUN HONGHE TOBACCO (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

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Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] ——Test tube slant culture

[0026] A Luria-Bertani (LB) medium was used, and the formula was: 10 g of peptone, 10 g of sodium chloride, 5 g of yeast extract, 20 g of agar, and distilled water to a volume of 1000 ml. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate Van35, culture at 28°C for 24 hours, and obtain test tube seeds.

Embodiment 2

[0028] - Seed cultivation

[0029] A Luria-Bertani (LB) medium was used, and the formula was: 10 g of peptone, 10 g of sodium chloride, 5 g of yeast extract, and distilled water to a volume of 1000 ml. Sterilize at 121°C for 25 minutes. Inoculate the Van35 test tube species and culture at 28°C for 12 hours to obtain liquid strains.

Embodiment 3

[0031] - Preparation of microbial preparations

[0032] Take the liquid strain, inoculate it into 200ml of LB liquid medium according to the inoculation amount of 0.1%, and cultivate it in a shaker at 37°C with a rotation speed of 180r / min until the OD value = 2.0. Centrifuge at 3500rpm / min for 10min, wash with sterile water Precipitate, centrifuge, then oscillate evenly with 20mL sterile water, and dilute 10 times when used to be the bacterial agent.

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Abstract

The invention discloses a microbial preparation for tobacco, a preparation method and application thereof, and belongs to the technical field of tobacco additives. The present invention obtains corresponding microbial preparations after liquid fermentation and culture of production strains. The production strain is Bacillus pumilus.Van35, which was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on November 5, 2009, with the preservation number CGMCC No.3411. The microbial preparation of the present invention can significantly improve the aroma and pleasantness of shredded tobacco, reduce miscellaneous gas and irritation, reduce smoke concentration and strength, and make the aftertaste more comfortable, thereby achieving the purpose of improving the smoking quality of shredded tobacco.

Description

technical field [0001] The invention belongs to the technical field of cigarette additives, and more specifically, the invention relates to a microbial preparation. At the same time, it also relates to the preparation method of the microbial preparation and its application in the tobacco industry. Background technique [0002] Tobacco is one of the important pillars of our country's finances. The State Tobacco Monopoly Administration clearly stated in Article 7 of the "Eleventh Five-Year" China Cigarette Science and Technology Development Outline that it is necessary to "overcome the key technologies that affect the aroma and quality of tobacco leaves" and narrow the quality gap with foreign tobacco leaves to improve domestic and foreign market competitiveness. . [0003] The main components in tobacco are carbohydrates and nitrogenous compounds. The former mainly includes sugar, starch, cellulose, lignin, hemicellulose, pectin and other cell wall components, while the la...

Claims

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Application Information

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IPC IPC(8): C12N1/20A24B15/20C12R1/07
Inventor 段焰青陈兴蒋举兴何华李庆华杨丽萍叶灵王文元李先毅
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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