Method for quantificationally detecting olefinic-bond-containing substance

A quantitative detection method and olefinic bond technology, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of cumbersome operation, expensive measurement, frequent sampling, etc., and achieve the effect of optimizing the fermentation process and technology

Inactive Publication Date: 2012-01-04
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This will inevitably lead to the need for frequent sampling when measuring the total isoprene production by chromatography, which is cumbersome to operate, and the chromatography itself also has the disadvantages of troublesome sample handling and expensive measurement.

Method used

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  • Method for quantificationally detecting olefinic-bond-containing substance
  • Method for quantificationally detecting olefinic-bond-containing substance

Examples

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Effect test

Embodiment 1

[0026] Example 1: Detection of liquid isoprene samples by potassium permanganate colorimetric method

[0027] Three liquid isoprene samples with known concentrations (40, 80, 120 mg / l) were added to potassium permanganate standard solution (1 g / l, pH=12) respectively. Shake and mix at room temperature for 10 minutes, centrifuge the above solution at 13,000 rpm for 2 minutes, dilute the supernatant 20 times, measure the absorbance at 546 nm, and calculate the concentration of isoprene according to the regression equation. Six replicates were performed for each sample to determine the precision of the method (relative standard deviation %RSD). The accuracy of the method (relative error %RE) is obtained by comparing the measured value with the actual value. The regression equation is: C=-359.71A+280.43 (C: detectable isoprene concentration, mg / l, 20mg / l

Embodiment 2

[0028] Example 2: Detection of isoprene in fermentation tail gas by potassium permanganate colorimetric method

[0029] The temperature of the fermentation process is 37°C. Due to the low boiling point of isoprene (34°C), the isoprene produced during the fermentation process volatilizes into the fermentation tail gas. 1 / 30 of the tail gas is used for measurement through a gas flow meter, and the branch exhaust pipe is immersed in a potassium permanganate standard solution, and is fermented at different times (4h, 6h, 8h, 10h, 12h, 14h, 16h, 20h, 24h , 28h, 32h, 36h, 40h, 44h, 48h) take 1ml of potassium permanganate solution, centrifuge the above solution at 13000rpm for 2 minutes, dilute the supernatant 20 times and measure the absorbance at 546nm, and calculate the isoprene according to the regression equation concentration of alkenes. The regression equation is: C=-359.71A+280.43 (C: detectable isoprene concentration, mg / l, 20mg / l

Embodiment 3

[0032] Embodiment 3: utilize potassium permanganate colorimetric method to detect cyclohexene

[0033] A cyclohexene sample was added to a potassium permanganate standard solution (1 g / l, pH=12). Shake and mix at room temperature for 10 minutes, centrifuge the above solution at 13,000 rpm for 2 minutes, dilute the supernatant 20 times, measure the absorbance at 546 nm, and calculate the concentration of cyclohexene according to the regression equation. The regression equation is: C=-671.14A+503.40 (C: detectable concentration of cyclohexene, mg / l, 10mg / l

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Abstract

The invention discloses a method for quantificationally detecting an olefinic-bond-containing substance. The method comprises the following steps of: oxidizing an olefinic bond into vicinal diol by using potassium permanganate which is taken as an oxidant, wherein the potassium permanganate is reduced and an absorption value of the potassium permanganate at the wavelength of 546nm is reduced at the same time; and calculating the content of the olefinic-bond-containing substance by the reduced absorbance by measuring an absorption value of reacted potassium permanganate solution at the wavelength of 546nm. By the method, the total amount of the olefinic-bond-containing substance can be truly showed, and only the approximate total amount of concentration integral of the olefinic-bond-containing substance is obtained by integral-based chromatography; and the sampling can be performed every several hours according to the actual demand and is more convenient and quicker than the sampling which is performed every several minutes by the traditional chromatography.

Description

technical field [0001] The invention relates to a quantitative detection method for substances containing ethylenic bonds. technical background [0002] Olefinic double bonds exist in a variety of natural compounds and active ingredients, which are important platform chemicals and widely used in a variety of fields. Biosynthesis of olefins such as isoprene, ethylene, and styrene oxide from renewable biomass feedstocks is a current research hotspot. Due to the complexity of the biological reaction process, it is necessary to create a suitable analytical method to quantify these bio-based chemicals, so as to have a better evaluation of the yield, yield, production intensity and other parameters of the fermentation process. At present, the analysis methods for ethylenic bond-containing substances such as isoprene mainly include high-performance liquid chromatography (HPLC) and gas chromatography (GC). These methods can sensitively measure the concentration of isoprene in liqui...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 咸漠秦博
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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