Weigela florida cv. Red-prince anther-inducing pollen plant culture medium

Abstract

A Weigela florida cv. Red-prince anther-inducing pollen plant culture medium, which relates to the technical field of plant tissue culture, is a special-purpose culture medium for Weigela florida cv. Red-prince anther callus induction, and solves the problem on how to obtain a pure line-breeding material for Weigela florida cv. Red-prince, the pure line of the Weigela florida cv. Red-prince is obtained by a way of anther culture, and thereby the process of breeding the Weigela florida cv. Red-prince as an excellent greening tree species can be accelerated. The Weigela florida cv. Red-prince anther-inducing pollen plant culture medium comprises MS culture medium, hormone and a carbon source. The invention adopts the Weigela florida cv. Red-prince anther as an explant, a method integrated with lighting culture and dark culture is adopted for alternate culture, the induction of anther callus is carried out, and thereby a pollen plant is obtained. The culture medium disclosed by the invention has the advantages that: callus proliferation is notable, the pollen plant can be easily formed, consequently, the Weigela florida cv. Red-prince-breeding speed is increased, and the breeding period is shortened.

Description

Technical field: [0001] The invention belongs to the technical field of plant tissue culture, and is a special culture medium for Hongzijindai anther-induced pollen plants. Background technique: [0002] In the nursery stock market in northern my country, the plant species and colors are relatively monotonous compared with those in the south, and there are few varieties of flowering shrubs suitable for planting in the northeast. The variety of landscaping in northern my country can improve the color level and grade of urban greening. Red-prince Jindai (Weigela florida cv.'Red-prince') is a deciduous shrub of the Lonicera family Lonicera genus, a variety of Weigela florida cv. 'Red-prince', with a plant height of 1.5-2.0m, a crown width of 1.5m, and leaves elliptical to Oval oblong, carmine corolla, long flowering period, light-loving, shade-tolerant, cold-resistant, not strict on soil, strong adaptability and stress resistance, can be planted alone or in clusters in the gard...

AI Technical Summary

Problems solved by technology

[0005] The invention solves the problem of obtaining pure-line breeding materials for the red prince brocade, thereby accelerating the breeding process of the excellent greening tree species red prince brocade, and laying the foundation for the breeding work of the red prince brocade

[0006] The present invention starts with screening pollen development period, basic culture medium, adjusting plant growth regulator and carbon source, rationally proportioning various hormones and carbon sources, and aims to provide a special culture medium for inducing pollen callus of Red Prince Jindai , to resolve induced pollen plants

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