Weigela florida cv. Red-prince anther-inducing pollen plant culture medium
A technology of red prince brocade and culture medium, which is applied in the field of plant tissue culture, can solve the problems of accelerating the breeding process of red prince brocade, achieve the effect of obvious callus proliferation and speed up breeding
Inactive Publication Date: 2012-01-11
FORESTRY RES INST OF HEILONGJIANG PROVINCE
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Problems solved by technology
[0005] The present invention aims to solve the problem of obtaining pure-line breeding materials for Red Prince Brocade, thereby accelerating the breeding process of excellent greening tree species Red Prince Brocade, and laying the foundation for the breeding work of Red Prince Brocade
[0006] The present invention starts with screening pollen development period, basic culture medium, adjusting plant growth regulator and carbon source, rationally proportioning various hormones and carbon sources, and aims to provide a special culture medium for inducing pollen callus of Red Prince Jindai , to resolve induced pollen plants
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[0028] The technical funds of the present invention come from the key scientific and technological research project of the Heilongjiang Provincial Department of Science and Technology "Research on the High-efficiency Propagation Technology of Excellent Landscape Tree Species" (GB07B303-02). After 2 years of research, the research group used the anthers of C. chinensis as explants to induce anther callus, and obtained pollen plants by alternating light and dark cultures.
[0029] Material collection and processing: Red Prince Jindai anthers were collected from the precious tree species garden of Heilongjiang Forest Botanical Garden. The mother tree is 3 years old and grows robustly. Flower bud collection is carried out in the growing season from June to August, when the pollen is in a period of vigorous growth, and the collection time is selected in sunny weather at 9-10 am. Flower bud collection The flower buds were collected every 2 days from bud sprouting to flowering, and a...
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A Weigela florida cv. Red-prince anther-inducing pollen plant culture medium, which relates to the technical field of plant tissue culture, is a special-purpose culture medium for Weigela florida cv. Red-prince anther callus induction, and solves the problem on how to obtain a pure line-breeding material for Weigela florida cv. Red-prince, the pure line of the Weigela florida cv. Red-prince is obtained by a way of anther culture, and thereby the process of breeding the Weigela florida cv. Red-prince as an excellent greening tree species can be accelerated. The Weigela florida cv. Red-prince anther-inducing pollen plant culture medium comprises MS culture medium, hormone and a carbon source. The invention adopts the Weigela florida cv. Red-prince anther as an explant, a method integrated with lighting culture and dark culture is adopted for alternate culture, the induction of anther callus is carried out, and thereby a pollen plant is obtained. The culture medium disclosed by the invention has the advantages that: callus proliferation is notable, the pollen plant can be easily formed, consequently, the Weigela florida cv. Red-prince-breeding speed is increased, and the breeding period is shortened.
Description
technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a special medium for inducing pollen plants from the anthers of red prince broccoli. Background technique [0002] In the nursery stock market in northern my country, the plant species and colors are more monotonous than those in the south, and there are few varieties of flowering shrubs suitable for planting in the northeast. In order to make up for this shortcoming, garden workers have introduced red prince brocade from the United States in recent years to enrich the market. Varieties of landscaping in northern my country, improve the color level and grade of urban greening. Weigela florida cv. 'Red-prince') is a genus of honeysuckle, a deciduous shrub, a varietal of daisy, with a plant height of 1.5-2.0m, a crown width of 1.5m, and leaves oval to Oval long oval shape, corolla carmine red, long flowering period, it likes light, shade tolerance, cold tolerance, not st...
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IPC IPC(8): A01H4/00
Inventor 李晶王承义王丹孙海滨舒钰刘强孙一萌刘维斌王晓荣王颖
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE