Microbial fermentation enzymolysis method for extracting grease and protein of soybean
A technology of microbial fermentation and soybean oil, which is applied in the field of soybean oil and protein extraction through microbial fermentation liquid enzymolysis, soybean oil and protein extraction, can solve the problem of increasing the complexity of the process, not getting rid of the use of organic solvents, and low actual yield problems such as reducing the workload of water washing to remove salt, reducing the workload of process operations, and reducing the use of alkali
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experiment example 1
[0054] 1 Materials and methods
[0055] 1.1 Experimental materials
[0056] 1.1.1 Strains
[0057] Bacillus subtilis was purchased from China Industrial Microbiology Collection Center (ATCC20524).
[0058] 1.1.2 Reagents
[0059] Beef extract, peptone, glucose, soluble starch, maltose, sucrose, tyrosine, folin phenol reagent, trichloroacetic acid, anhydrous sodium carbonate, and sodium chloride are all analytically pure; Tris-Hcl buffer solution (pH8. 9 is suitable for the determination of alkaline protease activity, pH 7.2 is suitable for the determination of neutral protease activity).
[0060] 1.1.3 Raw materials
[0061] Low-temperature defatted soybean meal: purchased from Harbin Gaoke Biotechnology Company.
[0062] 1.1.4 Medium
[0063] Incline preservation medium: 1% peptone, 0.5% beef extract, 0.5% sodium chloride, 1.5% agar, pH 7.5, sterilized at 121°C for 20min.
[0064] Seed medium: peptone 1%, beef extract 0.5%, sodium chloride 0.5%, pH 7.4, sterilized at 1...
experiment example 2
[0145] 1 Materials and methods
[0146] 1.1 Experimental materials
[0147] Raw materials: extruded soybeans.
[0148] 1.2 Extrusion and expansion: the moisture content of the material is 13%-17wt%, the sleeve temperature of the extrusion extruder is 85-110°C, and the screw speed is 95-115r / min.
[0149] 1.3 Crush: Crush the extruded material and pass it through a 70-120 mesh sieve.
[0150] 1.4 Experimental method
[0151] 1.4.1 Process flow
[0152] like Figure 19 shown.
[0153] 1.4.2 Test method:
[0154] Determination of protein content: Kjeldahl method
[0155] Determination of fat content in milk: Gothic-Ross method
[0156] Grease content in residue: Soxhlet extraction
[0157] 1.4.3 Test formula
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[0164] 1.4.4 Determination of the process conditions for extracting oil from fermented liquid hydrolyzed puffed soybean powder
[0165] Under the optimized medium and fermentation conditions,...
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